AIRE anticorps (C-Term)
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- Antigène Voir toutes AIRE Anticorps
- AIRE (Autoimmune Regulator (AIRE))
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Épitope
- C-Term
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Reactivité
- Humain
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Hôte
- Chèvre
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp AIRE est non-conjugé
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Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Chromatin Immunoprecipitation (ChIP)
- Séquence
- C-QSMARPAAPF PS
- Specificité
- This antibody detects AIRE / APECED at C-term. Expected to recognize isoforms 1 and 2 (NP_000374.1 and NP_000649.1 respecively).
- Réactivité croisée (Details)
- Species reactivity (tested):Human.
- Purification
- Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.
- Immunogène
- Peptide from C-Terminus of the protein sequence according to NP_000374.1, NP_000649.1. Genename: AIRE
- Top Product
- Discover our top product AIRE Anticorps primaire
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- Indications d'application
- Peptide ELISA: Limit Dilution: 1/32000. Western blot: 0.01-0.03 μg/mL. Appr.60 kDa in transfected HEK293 lysates (calculated MWof 57.0 kDa according to NP_000374.1). Immunohistochemistry: 2.5 μg/mL. In paraffin embedded Human Thymic medula showsstaining of select nuclei.
- Restrictions
- For Research Use only
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- Concentration
- 0.5 mg/mL
- Buffer
- Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
- Avoid repeated freezing and thawing.
- Stock
- 4 °C/-20 °C
- Stockage commentaire
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Antigène
- AIRE (Autoimmune Regulator (AIRE))
- Autre désignation
- AIRE / APECED (AIRE Produits)
- Synonymes
- anticorps AIRE1, anticorps APECED, anticorps APS1, anticorps APSI, anticorps PGA1, anticorps autoimmune regulator, anticorps autoimmune regulator (autoimmune polyendocrinopathy candidiasis ectodermal dystrophy), anticorps AaeL_AAEL013794, anticorps CpipJ_CPIJ001208, anticorps CpipJ_CPIJ001209, anticorps AIRE, anticorps Aire
- Sujet
- AIRE is a transcriptional regulator that is thought to be important in controlling tissue-specific expression of genes in the thymus. The precise function of the protein is not well defined. It contains zinc finger motifs and isoform 1 is localized to both the nucleus and cytoplasm. Three splice variant mRNAs products have been described. The longer AIRE1 mRNA appears to be more abundant and includes exons 1 through 14. Splice variant AIRE2 includes a portion of the non-coding region of exon 1, an alternatively spliced longer exon 8, plus exons 9 through 14. Variant AIRE3 includes the same exon 1-8-9 sequences as found in AIRE2 but utilizes additional alternative splicing in exon 10 that shifts the reading frame such that a stop codon in exon 12 is utilized. The resulting protein products of these splice variants differ significantly. Defects in this gene cause the autosomal-recessive systemic autoimmune disease termed autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy (APECED).Synonyms: Autoimmune polyendocrinopathy candidiasis ectodermal dystrophy protein, Autoimmune regulator
- ID gène
- 326
- NCBI Accession
- NP_000374
- UniProt
- O43918
- Pathways
- Chromatin Binding
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