LIME anticorps (C-Term)
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- Antigène Voir toutes LIME (LIME1) Anticorps
- LIME (LIME1) (Lck Interacting Transmembrane Adaptor 1 (LIME1))
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Épitope
- C-Term
- Reactivité
- Souris, Rat
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Hôte
- Chèvre
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp LIME est non-conjugé
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Application
- Western Blotting (WB)
- Séquence
- C-ENVYESIKEM GL
- Specificité
- This antibody recognizes LIME1.
- Réactivité croisée (Details)
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Species reactivity (expected):Rat.
Species reactivity (tested):Mouse - Purification
- Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.
- Immunogène
- Peptide from the C Terminus of the protein sequence according to NP_076173.1.
- Top Product
- Discover our top product LIME1 Anticorps primaire
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- Indications d'application
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Peptide ELISA: 1/128000. Western Blot: 0.5-2.0 μg/mL, Approx 32 kDa band observed in Mouse Heart lysates(predicted MW of 32 kDa according to NP_076173.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 0.5 mg/mL
- Buffer
- Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
- Avoid repeated freezing and thawing.
- Stock
- 4 °C/-20 °C
- Stockage commentaire
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Antigène
- LIME (LIME1) (Lck Interacting Transmembrane Adaptor 1 (LIME1))
- Autre désignation
- LIME1 (LIME1 Produits)
- Synonymes
- anticorps LIME1, anticorps DKFZp459A158, anticorps LIME, anticorps LP8067, anticorps dJ583P15.4, anticorps 2810038K19Rik, anticorps RGD1562795, anticorps Lck interacting transmembrane adaptor 1, anticorps LIME1, anticorps Lime1
- Sujet
- LIME (Lck-interacting molecule) is a new raft-associated adaptor protein involved in regulation of T cell activation by coreceptors. LIME becomes tyrosine phosphorylated after cross-linking of the CD4 or CD8 coreceptors. Phospho-LIME associates with the Src family kinase Lck and its negative regulator, Csk. Ectopic expression of LIME in Jurkat T cells results in an increase of Csk in lipid rafts, increased phosphorylation of Lck and higher Ca2+ response to CD3 stimulation.Synonyms: LIME, LIME-1, LIME1, Lck-interacting membrane protein, Lck-interacting molecule, Lck-interacting transmembrane adapter 1
- ID gène
- 54923
- NCBI Accession
- NP_060276
- UniProt
- Q9H400
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