NPM1 anticorps (C-Term)

N° du produit ABIN374536

Cet anticorps Chèvre Polyclonal détecte spécifiquement NPM1 dans WB et EIA. Il présente une réactivité avec des échantillons de Humain, Souris, Rat, Chien et Chimpanzé.

Aperçu rapide pour NPM1 anticorps (C-Term) (ABIN374536)

Antigène: NPM1 (Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1))

Reactivité: Humain, Souris, Rat, Chien, Chimpanzé

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp NPM1 est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Détail du produit anti-NPM1 anticorps

Épitope: C-Term

Séquence: C-QEAIQDLWQW RKSL

Specificité: This antibody recognizes Nucleophosmin.

Réactivité croisée (Details): Species reactivity (expected):Canine, Bovine.
Species reactivity (tested):Human, Mouse, Rat.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide

Immunogène: Peptide with sequence from the C Terminus of the protein sequence according to NP_002511.1, NP_954654.1.

Information d'application

Indications d'application: Peptide ELISA: 1/2000. Western Blot: 0.1 - 0.3 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3 containing 0.02 % Sodium Azide and 0.5 % BSA

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur NPM1

Antigène: NPM1 (Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1))

Autre désignation: Nucleophosmin

Sujet: Nucleophosmin (NPM) also called B23, nutramin and NO38 is a ubiquitously expressed phosphoprotein involved in ribosome assembly/transport, cytoplasmic/nuclear trafficking, regulation of DNA polymerase alpha activity, centrosome duplication, and regulation of p53. It is involved in the acute response of mammalian cells to environmental stress, such as UV rays. NPM continuously shuttles between the nucleus and cytoplasm. It has been shown to bind nucleic acid, prevent protein aggregation via its chaperone activities, protect enzymes during thermal denaturation, and facilitate renaturation of chemically denatured proteins. In its cellular structure role, there is evidence suggesting NPM is associated with the centrosome. It is the substrate of CDK2/cyclin E during duplication of centrosomes (cellular division). Because of NPM gene interaction with several tumor-associated chromosome translocations, NPM is thought to be a portion of several fusion proteins: NPM-ALK, NPM-RAR, and NPM-MLF1. While it is not thought to be part of the transforming potential of these fusion proteins, it is believed to act as the interface for oligomerization and oncogenic conversion of these tumor promoting fusion proteins.Synonyms: NPM, NPM1, Nucleolar Phosphoprotein B23, Nucleolar Protein NO38, Numatrin

ID gène: 4869

NCBI Accession: NP_002511

UniProt: P06748

Pathways: Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly