This antibody is specific to GAPDH (Internal). GAPDH is constitutively expressed in almost all tissues at high levels. It is therefore a useful marker when a Loading/Positive Control is required in Western blotting.
Réactivité croisée (Details)
Species reactivity (expected):Canine (Dog). Species reactivity (tested):Human, Mouse, Rat.
Purification
Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.
Immunogène
Peptide with sequence from the internal region of the protein sequence according to NP_002037.2. Genename: GAPDH
Peptide ELISA: 1/4000 (Antibody detection limit dilution). Western Blot: 0.1-0.3 μg/mL. A approx. 36 kDa band is observed in Human Liver, Testes and Tonsillysates and in Mouse Spleen lysates. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Schillace, Miller, Pisenti, Grotzke, Swarbrick, Lewinsohn, Carr: "A-kinase anchoring in dendritic cells is required for antigen presentation." dans: PLoS ONE, Vol. 4, Issue 3, pp. e4807, (2009) (PubMed).
Carr, Vugler, Lawrence, Chen, Ahmado, Chen, Semo, Gias, da Cruz, Moore, Walsh, Coffey: "Molecular characterization and functional analysis of phagocytosis by human embryonic stem cell-derived RPE cells using a novel human retinal assay." dans: Molecular vision, Vol. 15, pp. 283-95, (2009) (PubMed).
Vugler, Carr, Lawrence, Chen, Burrell, Wright, Lundh, Semo, Ahmado, Gias, da Cruz, Moore, Andrews, Walsh, Coffey: "Elucidating the phenomenon of HESC-derived RPE: anatomy of cell genesis, expansion and retinal transplantation." dans: Experimental neurology, Vol. 214, Issue 2, pp. 347-61, (2008) (PubMed).
Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is a metabolic enzyme originally thought to be only involved in Glycolysis. GAPDH is constitutively expressed at high levels in almost all tissues and serves therefore as a useful marker for the measurement of equal loading in western blotting experiments. Because of its ubiquitous expression, GAPDH is often seen as a housekeeping gene. However in the last two decades, every year a new function for this enzyme has been revealed. Besides its central role as a cytoplasmic enzyme in Glycolysis, recent evidence suggests that mammalian GAPDH is also involved in a great number of intracellular processes such as DNA replication and repair, nuclear membrane fusion, endocytosis, nuclear translocation in apoptosis, microtubule bundling, vesicular secretory transport, maintenance of telomere structure, phosphotransferase activity and nuclear RNA export. We now know that GAPDH is not only in the cytoplasm, but it shuttles between the cytoplasm and the nucleus and membranes. A lot of reports demonstrate the role of GAPDH in different pathologies such as progression in prostate cancer and ovarian cancer (mRNA stability), programmed neuronal cell death, and agerelated neuronal diseases. For example the sub-cellular distribution of GAPDH is abnormal in patients with Alzheimer's disease.Synonyms: CDABP0047, GAPD, Glyceraldehyde-3-Phosphate Dehydrogenase