AIRE
Reactivité: Humain, Souris
IF
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
Peptide ELISA: 1/32000. Western blot: 0.5-1.0 μg/mL. Immunohistochemistry: 2.5 μg/mL. In paraffin embedded Human Thymus shows nuclear staining in select thymocytes.
Restrictions
For Research Use only
Concentration
0.5 mg/mL
Buffer
Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Avoid repeated freezing and thawing.
Stock
4 °C/-20 °C
Stockage commentaire
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C.
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Ströbel, Hartmann, Rosenwald, Kalla, Ott, Friedel, Schalke, Kasahara, Tomaru, Marx: "Corticomedullary differentiation and maturational arrest in thymomas." dans: Histopathology, Vol. 64, Issue 4, pp. 557-66, (2014) (PubMed).
Jüngling, Liu, Lesting, Coulon, Sosulina, Reinscheid, Pape: "Activation of neuropeptide S-expressing neurons in the locus coeruleus by corticotropin-releasing factor." dans: The Journal of physiology, Vol. 590, Issue Pt 16, pp. 3701-17, (2012) (PubMed).
Wei, Akerman, Newey, Pan, Clinch, Jacob, Shen, Wilkins, Ellory: "The potassium-chloride cotransporter 2 promotes cervical cancer cell migration and invasion by an ion transport-independent mechanism." dans: The Journal of physiology, Vol. 589, Issue Pt 22, pp. 5349-59, (2011) (PubMed).
Yuan, Zhu, Zhou, Chen, Zhu, Ma, He, Tian, Shi: "Role of mast cell activation in inducing microglial cells to release neurotrophin." dans: Journal of neuroscience research, Vol. 88, Issue 6, pp. 1348-54, (2010) (PubMed).
Zelano, Berg, Thams, Hailer, Cullheim: "SynCAM1 expression correlates with restoration of central synapses on spinal motoneurons after two different models of peripheral nerve injury." dans: The Journal of comparative neurology, Vol. 517, Issue 5, pp. 670-82, (2009) (PubMed).
AIRE is a transcriptional regulator that is thought to be important in controlling tissue-specific expression of genes in the thymus. The precise function of the protein is not well defined. It contains zinc finger motifs and isoform 1 is localized to both the nucleus and cytoplasm. Three splice variant mRNAs products have been described. The longer AIRE1 mRNA appears to be more abundant and includes exons 1 through 14. Splice variant AIRE2 includes a portion of the non-coding region of exon 1, an alternatively spliced longer exon 8, plus exons 9 through 14. Variant AIRE3 includes the same exon 1-8-9 sequences as found in AIRE2 but utilizes additional alternative splicing in exon 10 that shifts the reading frame such that a stop codon in exon 12 is utilized. The resulting protein products of these splice variants differ significantly. Defects in this gene cause the autosomal-recessive systemic autoimmune disease termed autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy (APECED).Synonyms: Autoimmune polyendocrinopathy candidiasis ectodermal dystrophy protein, Autoimmune regulator