GTPase NRas anticorps (Internal Region)
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- Antigène Voir toutes GTPase NRas (NRAS) Anticorps
- GTPase NRas (NRAS)
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Épitope
- Internal Region
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Reactivité
- Humain, Rat, Souris, Chien
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Hôte
- Chèvre
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp GTPase NRas est non-conjugé
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Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Séquence
- C-RMKKLNSSDD GTQ
- Specificité
- This antibody detects NRAS.
- Réactivité croisée (Details)
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Species reactivity (expected):Mouse, Rat and Canine (Dog).
Species reactivity (tested):Human. - Purification
- Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.
- Immunogène
- Synthetic peptide corresponding to internal sequence of Human NRAS (NP_002515.1). Genename: NRAS
- Top Product
- Discover our top product NRAS Anticorps primaire
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- Indications d'application
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Peptide ELISA: Antibody detection limit dilution 1/32000. Western Blot: 0.01-0.03 μg/mL. Approx.21 kDa band observed in Human lysates of cell lineA431. In transfected HEK293 transiently expressing NRAS bands of approx.21 kDa and21 kDa are observed. These bands are not observed in the non-transfected HEK293. Immunohistochemistry: 2-3 μg/mL. In paraffin embedded Human Small Intestine shows stainingof cytoplasm and membrane of enterocytes.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 0.5 mg/mL
- Buffer
- Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
- Avoid repeated freezing and thawing.
- Stock
- 4 °C/-20 °C
- Stockage commentaire
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Antigène
- GTPase NRas (NRAS)
- Autre désignation
- NRAS (NRAS Produits)
- Synonymes
- anticorps NRAS, anticorps AV095280, anticorps N-ras, anticorps ALPS4, anticorps NRAS1, anticorps NS6, anticorps N-RAS, anticorps fj99a10, anticorps wu:fj99a10, anticorps zgc:100761, anticorps zras-b1, anticorps H-RAS, anticorps HRAS, anticorps GTPase NRas, anticorps neuroblastoma RAS viral (v-ras) oncogene homolog L homeolog, anticorps NRAS proto-oncogene, GTPase, anticorps neuroblastoma ras oncogene, anticorps neuroblastoma RAS viral oncogene homolog, anticorps Tsp_01117, anticorps nras.L, anticorps Nras, anticorps NRAS, anticorps nras
- Sujet
- NRAS is an oncogene encoding a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.Synonyms: ALPS4, GTPase NRas, HRAS1, N-Ras, Transforming protein N-Ras, neuroblastoma RAS viral (v-ras) oncogene homolog
- Poids moléculaire
- 21 kDa (NP_002515.1).
- ID gène
- 4893, 9606
- UniProt
- P01111
- Pathways
- Signalisation p53, Signalisation MAPK, Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Hepatitis C, Regulation of long-term Neuronal Synaptic Plasticity, VEGF Signaling
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