L’anticorps anti-EWSR1 Polyclonal Chèvre est utilisé pour la détection de EWSR1 dans des échantillons de Humain, Souris, Rat et Boeuf (Vache). Il a été validé pour WB, IHC (p) et EIA.
This antibody is expected to recognize all five reported isoforms.
Réactivité croisée (Details)
Species reactivity (expected):Bovine, Mouse, Rat. Species reactivity (tested):Human.
Purification
Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.
Immunogène
Peptide with sequence from the internal region of the protein sequence according to NP_053733.2, NP_005234.1, NP_001156757.1, NP_001156758.1, NP_001156759.1. Genename: EWSR1
EWSR1
Reactivité: Humain, Souris, Rat
WB, IHC, IF, ICC, FACS, IHC (fro)
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
Peptide ELISA: 1/16000. Western Blot: 1 - 3 μg/mL. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
0.5 mg/mL
Buffer
Tris saline, pH ~7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Avoid repeated freezing and thawing.
Stock
4 °C/-20 °C
Stockage commentaire
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Antigène
EWSR1
(Ewing Sarcoma Breakpoint Region 1 (EWSR1))
Autre désignation
EWSR1
Sujet
EWSR1 is a putative RNA binding protein of unknown function. Mutations in this gene, specifically at (11,22)(q24,q12) translocation, are known to cause Ewing sarcoma as well as neuroectodermal and various other tumors. Alternative splicing of this gene results in two products.Synonyms: EWS, EWS oncogene, EWSR-1, Ewing sarcoma breakpoint region 1 protein, RNA-binding protein EWS