MBD2 anticorps (Internal Region, Isoform 1)
-
- Antigène Voir toutes MBD2 Anticorps
- MBD2 (Methyl-CpG Binding Domain Protein 2 (MBD2))
-
Épitope
- Internal Region, Isoform 1
-
Reactivité
- Humain, Souris, Rat, Chien
-
Hôte
- Chèvre
-
Clonalité
- Polyclonal
-
Conjugué
- Cet anticorp MBD2 est non-conjugé
-
Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
- Séquence
- C-RNDPLNQNKG KPDLN
- Specificité
- This antibody is expected to resognize isoform 1 (NP_003918.1) only.
- Réactivité croisée (Details)
-
Species reactivity (expected):Mouse, Rat, Canine.
Species reactivity (tested):Human. - Purification
- Affinity Chromatography
- Immunogène
- Peptide with sequence from the internal region of the protein sequence Genename: MBD2
- Top Product
- Discover our top product MBD2 Anticorps primaire
-
-
- Indications d'application
-
Peptide ELISA: 1/64000. Western blot: Approx 48-50 kDa band observed in lysates of cell line Jurkat (calculated MWof 43.0 kDa according to NP_003918.1). The observed molecular weight corresponds toearlier findings in literature with different antibodies (Tan et al, Mol Cell Biol.2006Oct,26(19): 7224-35. , PMID: 16980624). Recommended concentration: 0.3 - 1 μg/mL. Immunohistochemistry on paraffin sections: Human Colon shows nuclear stianing ofenterocytes. Recommended concentration: 2 - 4 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
-
- Concentration
- 0.5 mg/mL
- Buffer
- Tris saline, 0.02 % Sodium Azide, pH 7.3 with 0.5 % BSA
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
- Avoid repeated freezing and thawing.
- Stock
- 4 °C/-20 °C
- Stockage commentaire
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
-
- Antigène
- MBD2 (Methyl-CpG Binding Domain Protein 2 (MBD2))
- Autre désignation
- MBD2 (MBD2 Produits)
- Synonymes
- anticorps MBD2, anticorps DMTase, anticorps NY-CO-41, anticorps MBD2a, anticorps wu:fa97f06, anticorps zgc:111862, anticorps methyl-CpG binding domain protein 2, anticorps methyl-CpG binding domain protein 2 S homeolog, anticorps MBD2, anticorps Mbd2, anticorps mbd2, anticorps mbd2.S
- Sujet
- DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. This protein may function as a mediator of the biological consequences of the methylation signal.Synonyms: DMTase, Demethylase, Methyl-CpG-binding domain protein 2
- ID gène
- 8932
- NCBI Accession
- NP_003918
- UniProt
- Q9UBB5
- Pathways
- Chromatin Binding
-