Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. ELISA: 0.05-0.2 µg/mL Western blot: 0.1-1.0 µg/mL Immunoprecipitation: 1 µg/mL Other applications: user-optimized
Restrictions
For Research Use only
Format
Lyophilized
Buffer
PBS, pH 7.4, containing 0.02 % Sodium azide
Agent conservateur
Sodium azide
Précaution d'utilisation
WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Conseil sur la manipulation
Avoid repeated freezing and thawing cycles.
Stock
4 °C/-20 °C
Stockage commentaire
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below.
Reuben, Phénix, Santos, Lapointe: "The WT hemochromatosis protein HFE inhibits CD8? T-lymphocyte activation." dans: European journal of immunology, Vol. 44, Issue 6, pp. 1604-14, (2014) (PubMed).
Maryon, Molloy, Kaplan: "Cellular glutathione plays a key role in copper uptake mediated by human copper transporter 1." dans: American journal of physiology. Cell physiology, Vol. 304, Issue 8, pp. C768-79, (2013) (PubMed).
The DYKDDDDK peptide is a small component of the epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors.