Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Purification
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Immunogène
This Apobec1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 7-36 amino acids from the N-terminal region of human Apobec1.
APOBEC1 is involved in the production of apolipoprotein B (apoB)-48 from apoB-100. The gene spans 18 kb and contains five exons, all of which are translated. Alternative splicing produces a variant transcript that lacks exon 2 and encodes a novel 36-amino acid peptide. The exon 2-skipped transcript accounts for approximately 50 % of APOBEC1 mRNA in the adult small intestine and up to 90 % of APOBEC1 mRNA in the developing gut. Exon 2-skipping may thus be a quantitatively important mechanism for regulating the expression of this gene in the gastrointestinal tract.