This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Immunogène
This NCAPH2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 198-227 amino acids from the N-terminal region of human NCAPH2.
NCAPH2
Reactivité: Humain
WB, ELISA
Hôte: Lapin
Polyclonal
APC
Indications d'application
WB: 1:1000
Restrictions
For Research Use only
Format
Liquid
Buffer
Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
4 °C,-20 °C
Stockage commentaire
Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Date de péremption
6 months
Lai, Wong, Lee, Li: "Caspase-3-mediated degradation of condensin Cap-H regulates mitotic cell death." dans: Cell death and differentiation, Vol. 18, Issue 6, pp. 996-1004, (2011) (PubMed).
Lisková, Susor, Pivonková, Sasková, Karabínová, Kubelka: "Detection of condensin I and II in maturing pig oocytes." dans: Reproduction, fertility, and development, Vol. 22, Issue 4, pp. 644-52, (2010) (PubMed).
Antigène
NCAPH2
(Non-SMC Condensin II Complex, Subunit H2 (NCAPH2))
anticorps MGC81656, anticorps CAPH2, anticorps si:dkey-202b22.2, anticorps non-SMC condensin II complex subunit H2, anticorps non-SMC condensin II complex subunit H2 L homeolog, anticorps non-SMC condensin II complex, subunit H2, anticorps NCAPH2, anticorps ncaph2.L, anticorps ncaph2, anticorps Ncaph2
Sujet
Structural maintenance of chromosomes (SMC) and non-SMC condensin proteins associate into complexes that have been implicated in the process of chromosome condensation. A crucial prerequisite for accurate segregation of replicated sister chromatids is the condensation of the chromosomes into a manageable form prior to metaphase. The condensin I complex consists of two SMC subunits, SMC2 and SMC4, and three non-SMC subunits, CAP-H, CAP-G, and CAP-D2. An alternative complex, the condensin II complex, contains alternate non-SMC subunits, CAP-G2, CAP-H2, and CAP-D3. CAP-H2 is also known as Non-SMC condensin II complex, subunit H2 (NCAPH2) or kleisin beta isoform 2. The three non-SMC subunits in the condensing complexes form a regulatory subcomplex that is required to activate the SMC ATPases and to promote mitosis-specific chromatin binding of the holocomplex. The precise individual functions of each non-SMC protein in activation remain to be determined.