Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Purification
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Immunogène
This ARFGAP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 377-406 amino acids from the C-terminal region of human ARFGAP1.
ARFGAP1
Reactivité: Humain, Singe
WB
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
WB: 1:8000. WB: 1:1000. IHC-P: 1:50~100
Restrictions
For Research Use only
Format
Liquid
Buffer
Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
4 °C,-20 °C
Stockage commentaire
Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Date de péremption
6 months
Nishimura, Uchida, Yachi, Kudlyk, Lupashin, Inoue, Taguchi, Arai: "Oxysterol-binding protein (OSBP) is required for the perinuclear localization of intra-Golgi v-SNAREs." dans: Molecular biology of the cell, Vol. 24, Issue 22, pp. 3534-44, (2013) (PubMed).
Zhang, Major, Takanashi, Camp, Nishiya, Peters, Ginsberg, Jian, Randazzo, Schultz, Moon, Ding: "Small-molecule synergist of the Wnt/beta-catenin signaling pathway." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 18, pp. 7444-8, (2007) (PubMed).
Antigène
ARFGAP1
(ADP-Ribosylation Factor GTPase Activating Protein 1 (ARFGAP1))
ARFGAP1 is a GTPase-activating protein (GAP) which associates with the Golgi apparatus and which interacts with ADP-ribosylation factor 1 (ARF1). The encoded protein promotes hydrolysis of ARF1-bound GTP and is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is required for the fusion of these vesicles with target compartments. The activity of this protein is stimulated by phosphoinosides and inhibited by phosphatidylcholine.