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Vimentin anticorps (C-Term)

Name: Vimentin anticorps (C-Term)
SKU: ABIN389212
Price: 467.5 EUR
Availability: InStock

VIM Reactivité: Humain WB, IF, FACS, IHC (p) Hôte: Lapin Polyclonal RB16691 unconjugated

Anti-Vimentin Antibody (C-term) at 1:1000 dilution + U-2OS whole cell lysate Lysates/proteins at 20 μg per lane. (Vimentin anticorps (C-Term))

Vimentin Antibody (C-term) (ABIN389212 and ABIN2839367) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. (Vimentin anticorps (C-Term))

IHC (p)

Fluorescent confocal image of SY5Y cells stained with Vimentin (C-term) antibody. (Vimentin anticorps (C-Term))

Western blot analysis of Vimentin antibody (C-term) (ABIN389212 and ABIN2839367) in NCI- cell line lysates (35 μg/lane). (Vimentin anticorps (C-Term))

Vimentin Antibody (C-term) (ABIN389212 and ABIN2839367) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram). (Vimentin anticorps (C-Term))

FACS

5 images

(2 références)

N° du produit ABIN389212

Fiche de données

Antigène Voir toutes Vimentin (VIM) Anticorps

Vimentin (VIM)
Épitope
32

17

16

16

11

10

9

7

4

3

3

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 430-457, C-Term
Reactivité
314

130

126

61

60

50

43

38

30

22

20

14

13

8

8

6

6

3

3

2

1

1

1

1

1

1

1

1

1
Humain
Hôte
167

130

11

6

6

5

2
Lapin
Anticorps secondaires appropriés
Clonalité
181

145
Polyclonal
Conjugué
201

15

13

12

9

7

3

3

3

3

3

3

3

3

3

3

3

3

3

3

3

3

3

3

2

2

2

2

2

2

2

1

1
Cet anticorp Vimentin est non-conjugé
Application
266

115

107

96

90

63

47

40

39

33

23

20

8

6

4

4

3

2

2

2

1

1

1

1

1

1

1

1

1
Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Homologie

X, B, C, Ha, Pr, M, Pig, Rat

Purification

This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogène

This Vimentin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 430-457 amino acids from the C-terminal region of human Vimentin.

Clone

RB16691

Isotype

Ig Fraction

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anti-Vimentin (VIM) (AA 2-466) antibody
VIM Reactivité: Humain WB, IHC, ELISA, FACS Hôte: Souris Monoclonal 3A1F2 unconjugated

Indications d'application

IF: 1:100. WB: 1:1000. WB: 1:1000. IHC-P: 1:10~50. FC: 1:10~50

Restrictions

For Research Use only
Format

Liquid

Buffer

Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Agent conservateur

Sodium azide

Précaution d'utilisation

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock

4 °C,-20 °C

Stockage commentaire

Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Date de péremption

6 months
Liu, Li, Li, Yang: "Pituitary tumor transforming gene PTTG2 induces psoriasis by regulating vimentin and E-cadherin expression." dans: International journal of clinical and experimental pathology, Vol. 8, Issue 9, pp. 10887-93, (2016) (PubMed).

Dai, Duan, Duan, Zhou, He, Tu, Shen: "Down-regulation of TCF21 by hypermethylation induces cell proliferation, migration and invasion in colorectal cancer." dans: Biochemical and biophysical research communications, (2015) (PubMed).
Antigène

Vimentin (VIM)

Autre désignation

Vimentin (VIM Produits)

Sujet

Along with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements. The subunits display a tissue-specific pattern of expression. Desmin is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.

Poids moléculaire

53652

ID gène

7431

NCBI Accession

NP_003371

UniProt

P08670

Pathways

Caspase Cascade in Apoptosis