This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogène
This Phospho-AKT1-pS473 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S473 of human AKT1.
AKT1
Reactivité: Humain, Souris, Rat, Singe
WB, ELISA, IHC, IP
Hôte: Souris
Monoclonal
17F6-B11
unconjugated
Indications d'application
WB: 1:1000. DB: 1:500
Restrictions
For Research Use only
Format
Liquid
Buffer
Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
4 °C,-20 °C
Stockage commentaire
Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Date de péremption
6 months
Hu, Zhou, Zhao, Wu: "Integrin α6/Akt/Erk signaling is essential for human breast cancer resistance to radiotherapy." dans: Scientific reports, Vol. 6, pp. 33376, (2018) (PubMed).
Li, Miao, Shan, Liu, Li, Zhao, Jia: "MiR-106b and miR-93 regulate cell progression by suppression of PTEN via PI3K/Akt pathway in breast cancer." dans: Cell death & disease, Vol. 8, Issue 5, pp. e2796, (2018) (PubMed).
Zhao, Shan, Liu, Li, Jia: "Functional screen analysis reveals miR-3142 as central regulator in chemoresistance and proliferation through activation of the PTEN-AKT pathway in CML." dans: Cell death & disease, Vol. 8, Issue 5, pp. e2830, (2018) (PubMed).
Miao, Zheng, Li, Su, Zhao, Zhou, Jia: "MicroRNA-130b targets PTEN to mediate drug resistance and proliferation of breast cancer cells via the PI3K/Akt signaling pathway." dans: Scientific reports, Vol. 7, pp. 41942, (2017) (PubMed).
Li, Lu, Yu, Han, Wang, Mao, Shen, Wang, Tang, Li, Song: "miR-221/222 promote cancer stem-like cell properties and tumor growth of breast cancer via targeting PTEN and sustained Akt/NF-κB/COX-2 activation." dans: Chemico-biological interactions, Vol. 277, pp. 33-42, (2017) (PubMed).
Ren, Jia, Li, Gong, Liu, Zhang, Wang, Zhao: "ST6GalNAcII mediates the invasive properties of breast carcinoma through PI3K/Akt/NF-?B signaling pathway." dans: IUBMB life, Vol. 66, Issue 4, pp. 300-8, (2014) (PubMed).
Li, Jia, Ren, Liu, Gong, Wang, Zhang, Zhao: "Axl mediates tumor invasion and chemosensitivity through PI3K/Akt signaling pathway and is transcriptionally regulated by slug in breast carcinoma." dans: IUBMB life, Vol. 66, Issue 7, pp. 507-18, (2014) (PubMed).
The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery.