Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Purification
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Immunogène
This GCN2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human GCN2.
EIF2AK4
Reactivité: Souris
WB, IHC (p)
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
WB: 1:1000. IHC-P: 1:50~100
Restrictions
For Research Use only
Format
Liquid
Buffer
Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
4 °C,-20 °C
Stockage commentaire
Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Date de péremption
6 months
Fougeray, Mami, Bertho, Beaune, Thervet, Pallet: "Tryptophan depletion and the kinase GCN2 mediate IFN-?-induced autophagy." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 189, Issue 6, pp. 2954-64, (2012) (PubMed).
Lu, László, Miao, Chen, Wu: "The role of nitric-oxide synthase in the regulation of UVB light-induced phosphorylation of the alpha subunit of eukaryotic initiation factor 2." dans: The Journal of biological chemistry, Vol. 284, Issue 36, pp. 24281-8, (2009) (PubMed).
GCN2 (EIF2AK4) belongs to a family of kinases that phosphorylate the alpha subunit of eukaryotic translation initiation factor-2 to downregulate protein synthesis in response to varied cellular stresses.