Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Homologie
M, Rat
Purification
This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogène
This HIPK3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1127-1156 amino acids from the C-terminal region of human HIPK3.
HIPK3
Reactivité: Humain
IF (cc), IF (p)
Hôte: Lapin
Polyclonal
AbBy Fluor® 488
Indications d'application
WB: 1:2000. WB: 1:1000. IHC-P: 1:25
Restrictions
For Research Use only
Format
Liquid
Buffer
Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
4 °C,-20 °C
Stockage commentaire
Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Date de péremption
6 months
Lan, Wu, Shih, Chung: "Death-associated protein 6 (Daxx) mediates cAMP-dependent stimulation of Cyp11a1 (P450scc) transcription." dans: The Journal of biological chemistry, Vol. 287, Issue 8, pp. 5910-6, (2012) (PubMed).
Tiefenbach, Moll, Nelson, Hu, Baev, Kislinger, Krause: "A live zebrafish-based screening system for human nuclear receptor ligand and cofactor discovery." dans: PLoS ONE, Vol. 5, Issue 3, pp. e9797, (2010) (PubMed).
Lan, Li, Lin, Lai, Chung: "Cyclic AMP stimulates SF-1-dependent CYP11A1 expression through homeodomain-interacting protein kinase 3-mediated Jun N-terminal kinase and c-Jun phosphorylation." dans: Molecular and cellular biology, Vol. 27, Issue 6, pp. 2027-36, (2007) (PubMed).
Antigène
HIPK3
(Homeodomain Interacting Protein Kinase 3 (HIPK3))
HIPK3 negatively regulates apoptosis by promoting FADD phosphorylation. This kinase enhances androgen receptor-mediated transcription, and may act as a transcriptional corepressor for NK homeodomain transcription factors.