This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Immunogène
This CA3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 17-46 amino acids from the N-terminal region of human CA3.
CA3
Reactivité: Rat
WB, IHC, ICC
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
WB: 1:1000. WB: 1:1000
Restrictions
For Research Use only
Format
Liquid
Buffer
Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
4 °C,-20 °C
Stockage commentaire
Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Date de péremption
6 months
Pollard, Shephard, Freed, Liddell, Chakrabarti: "Mitochondrial proteomic profiling reveals increased carbonic anhydrase II in aging and neurodegeneration." dans: Aging, Vol. 8, Issue 10, pp. 2425-2436, (2017) (PubMed).
Araujo, Vaz, Fujimura, Fonseca, de Lima, Canhão, Venturini, Cardozo, Carvalho, Napimoga, Goulart, Gonçalves, Ueira-Vieira: "Improved serological detection of rheumatoid arthritis: a highly antigenic mimotope of carbonic anhydrase III selected in a murine model by phage display." dans: Arthritis research & therapy, Vol. 17, pp. 168, (2016) (PubMed).
Carbonic anhydrase III (CAIII) is a member of carbonic anhydrase isozymes. These carbonic anhydrases are a class of metalloenzymes that catalyze the reversible hydration of carbon dioxide and are differentially expressed in a number of cell types. The expression of the CA3 gene is strictly tissue specific and present at high levels in skeletal muscle and much lower levels in cardiac and smooth muscle. A proportion of carriers of Duchenne muscle dystrophy have a higher CA3 level than normal.