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GAPDH anticorps (C-Term)

Name: GAPDH anticorps (C-Term)
SKU: ABIN392354
Price: 467.5 EUR
Availability: InStock

GAPDH Reactivité: Humain WB, IF, IHC (p), FACS Hôte: Lapin Polyclonal RB16543 unconjugated

Western blot analysis of GDH Antibody (C-term ) 7873b in , , CEM cell line lysates (35 μg/lane). (GAPDH anticorps (C-Term))

Flow cytometric analysis of HepG2 cells using GDH Antibody (C-term )(bottom histogram) compared to a negative control cell (top histogram). (GAPDH anticorps (C-Term))

FACS

Confocal immunofluorescent analysis of GDH Antibody (C-term ) 7873b with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). (GAPDH anticorps (C-Term))

All lanes : Anti-GDH Antibody (C-term ) at 1:1000 dilution Lane 1: A431 whole cell lysate Lane 2: Hela whole cell lysate Lysates/proteins at 20 μg per lane. (GAPDH anticorps (C-Term))

Western blot analysis of lysates from Hela,HUVEC cell line (from left to right),using GDH Antibody (C-term ) 7873b. (GAPDH anticorps (C-Term))

Formalin-fixed and paraffin-embedded human prostate carcinoma with GDH Antibody (C-term ), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. (GAPDH anticorps (C-Term))

IHC (p)

7 images

(1 reference)

N° du produit ABIN392354

Fiche de données

Antigène Voir toutes GAPDH Anticorps

GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))
Épitope
33

13

11

7

6

6

6

5

4

4

3

3

3

3

3

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 233-259, C-Term
Reactivité
193

129

112

49

44

38

24

24

22

19

19

19

17

15

14

14

10

9

8

6

4

4

4

4

4

4

4

3

3

2

2

2

2

1

1

1

1

1

1
Humain
Hôte
145

82

16

11

4
Lapin
Anticorps secondaires appropriés
Clonalité
168

88
Polyclonal
Conjugué
152

30

16

15

3

3

3

3

3

3

3

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Cet anticorp GAPDH est non-conjugé
Application
202

98

73

65

35

34

29

26

15

14

7

6

5

4

3

3

3

3

1

1

1

1
Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Homologie

C, M, Pig, Rat

Purification

This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogène

This GAPDH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 233-259 amino acids from the C-terminal region of human GAPDH.

Clone

RB16543

Isotype

Ig Fraction

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GAPDH Reactivité: Humain WB, IHC, IF, IP Hôte: Lapin Polyclonal unconjugated

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GAPDH Reactivité: Humain, Souris, Rat WB, IF, IHC (p) Hôte: Souris Monoclonal 1A10A10 unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (C-Term) antibody
Verified GAPDH Reactivité: Humain, Rat WB, ELISA, IHC, IF Hôte: Chèvre Polyclonal unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 226-335) antibody
GAPDH Reactivité: Humain WB, ELISA Hôte: Souris Monoclonal 3C2 unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (Internal Region) antibody
Verified GAPDH Reactivité: Humain, Souris, Rat WB, ELISA, IHC, IF Hôte: Chèvre Polyclonal unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 136-335) antibody
GAPDH Reactivité: Humain, Souris, Rat WB, IHC (p) Hôte: Lapin Polyclonal unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 62-91), (N-Term) antibody
GAPDH Reactivité: Humain WB, IF, IHC (p) Hôte: Lapin Polyclonal RB16537 unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 154-324) antibody
GAPDH Reactivité: Humain WB, IHC, IP, ICC Hôte: Lapin Polyclonal unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 2-148) antibody
GAPDH Reactivité: Humain WB, IHC, IP, ICC Hôte: Lapin Polyclonal unconjugated

Indications d'application

IF: 1:10~50. IF: 1:10~50. WB: 1:1000. WB: 1:1000. WB: 1:1000. IHC-P: 1:50~100. FC: 1:10~50

Restrictions

For Research Use only
Format

Liquid

Buffer

Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Agent conservateur

Sodium azide

Précaution d'utilisation

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock

4 °C,-20 °C

Stockage commentaire

Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Date de péremption

6 months
Wang, Chen, Wang, Yu, Zhao, Zhang, Tang, Peng: "Metalloproteases meprin-ɑ (MEP1A) is a prognostic biomarker and promotes proliferation and invasion of colorectal cancer." dans: BMC cancer, Vol. 16, pp. 383, (2017) (PubMed).
Antigène

GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Autre désignation

GAPDH (GAPDH Produits)

Sujet

GAPDH catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains.

Poids moléculaire

36053

ID gène

2597

NCBI Accession

NP_001243728, NP_002037

UniProt

P04406