Histone H2B anticorps (Lys20)

N° du produit ABIN1574118

Détail du produit anti-Histone H2B anticorps

Antigène: Histone H2B

Épitope: Lys20

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Histone H2B est non-conjugé

Application: Western Blotting (WB), ELISA

Specificité: Rabbit Anti-Histone H2B (Acetyl-K20) Polyclonal Antibody detects endogenous levels of human and mouse Histone H2B only when acetylated at Lys20. It is predicted to react with rat species according to sequence homology.Positive Control: Hela, HepG2 and mouse brain.

Réactivité croisée (Details): Rabbit Anti-Histone H2B (Acetyl-K20) Polyclonal Antibody detects endogenous levels of human and mouse Histone H2B only when acetylated at Lys20. It is predicted to react with rat species according to sequence homology.Positive Control: Hela, HepG2 and mouse brain.

Purification: Immunoaffinity chromatography using acetylpeptide, cross-adsorbed with the corresponding non-acetylpeptide

Immunogène: a KLH-coupled synthetic acetylpeptide corresponding - AA surrounding Lys20 of human Histone H2B. .

Isotype: IgG

Information d'application

Indications d'application: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

ELISA: 0.05-0.2 µg/mL
Western blot: 0.5-1 µg/mLOther Applications: user-optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: PBS, pH 7.4, containing 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Stock: 4 °C/-20 °C

Stockage commentaire: The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Détails sur Histone H2B

Antigène: Histone H2B

Abstract: Histone H2B Produits

Synonymes: anticorps Hist1h2bc, anticorps H2B, anticorps HISTONE H2B, anticorps MRN17.11, anticorps MRN17_11, anticorps histone B2, anticorps H2B1, anticorps HIS2B, anticorps histone cluster 1 H2B family member k, anticorps histone H2B, anticorps histone B2, anticorps histone H2B.3, anticorps Hist1h2bk, anticorps H2B, anticorps HTB2, anticorps THAPSDRAFT_27352, anticorps THAPSDRAFT_37309, anticorps THAPS_6947, anticorps THAPSDRAFT_34210, anticorps LOC426037, anticorps LOC107802329

Sujet: Histone H2B is a core component of nucleosome that facilitates nucleosome wrapping and DNA compacting into chromatin. This limits the accessibility of DNA to the cellular machineries for which DNA is required as a template. Histones thereby play a central role in transcription regulation, DNA repair and replication as well as chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, of which the process is called the histone code, and nucleosome remodeling.Rabbit Anti-Histone H2B (Acetyl-K20) Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic acetylpeptide corresponding to residues surrounding Lys20 of human Histone H2B. (Swiss Prot: Q5QNW6).