S100A1 anticorps

N° du produit ABIN401026

Détail du produit anti-S100A1 anticorps

Antigène: S100A1 (S100 Calcium Binding Protein A1 (S100A1))

Reactivité: Boeuf (Vache)

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp S100A1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificité: This antibody is directed against S100 protein.

Réactivité croisée (Details): Species reactivity (expected):Primate, Mouse, Rat, Canine.
Species reactivity (tested):Bovine.

Purification: Affinity chromatography on Protein A

Immunogène: Prepared from whole rabbit serum produced by repeated immunizations with fulllength bovine S100 protein (mixture of alphaalpha homodimers and alphabeta heterodimers)

Isotype: IgG

Information d'application

Indications d'application: ELISA: 1/5,000 - 1/25,000. Western Blot: 1/500 - 1/3,000. Immunohistochemistry: 1/200 - 1/2,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore with 0.1 mL of deionized water (or equivalent).

Concentration: 5.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Détails sur S100A1

Antigène: S100A1 (S100 Calcium Binding Protein A1 (S100A1))

Autre désignation: S100A1 (S100A1 Produits)

Synonymes: anticorps S100, anticorps S100-alpha, anticorps S100A, anticorps S100A1, anticorps DKFZp468K0511, anticorps AI266795, anticorps S100a, anticorps S100 calcium binding protein A1, anticorps S100A1, anticorps S100a1, anticorps s100a1

Sujet: S-100 protein derived from brain tissue is an acidic calcium-binding protein with molecular weight of about 21 kDa. In human brain tissue S-100 protein is mainly presented as two isoforms - betabeta homodimer (S-100b) and alphabeta heterodimer (S-100a). Because of its predominant location in astroglial cells S-100 protein can be used as a sensitive and reliable marker for central nervous system injury. Structural damage of glial cells causes leakage of S-100 protein into the extracellular matrix and into cerebrospinal fluid, further releasing into the bloodstream. Measurements of S-100 protein in patient serum samples are useful in monitoring of traumatic brain injury, ischemic brain damage after circulatory arrests, and in diagnosis and prognosis of clinical outcome in acute stroke. Although predominant among the water-soluble brain proteins, S-100 is also found in a variety of other tissues. S-100 is an intracellular protein that weakly binds calcium. It binds zinc very tightly, however, and this appears to increase the affinity of the protein for calcium. Distinct binding sites, with different affinities, exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites.Synonyms: S-100 protein alpha chain, S-100 protein subunit alpha, S100 alpha, S100 calcium-binding protein A1, S100-A1, S100A

ID gène: 528735

NCBI Accession: NP_001092512

UniProt: P02639

Pathways: Regulation of Muscle Cell Differentiation, Toll-Like Receptors Cascades, S100 Proteins