ASAP3
Reactivité: Souris, Rat
WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro)
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
ELISA: 1/1,000 - 1/5,000. Western Blot: 1/1000 - 1/10,000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
1.10 mg/mL (by UV absorbance at 280 nm)
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Avoid repeated freezing and thawing.
Stock
-20 °C
Antigène
ASAP3
(ArfGAP with SH3 Domain, Ankyrin Repeat and PH Domain 3 (ASAP3))
UPLC1 (up-regulated in liver cancer 1), also named DDEFL1 (development and differentiation-enhancing factor-like 1) or ASAP3, is a member of the AZAP family of proteins. These proteins catalyze the hydrolysis of GTP bound to ADP-ribosylation factor (Arf) proteins, thereby causing Arf inactivation. For this reason, the ASAPs are generally called ArfGAPs. The activity of ArfGAPs is dependent on the presence of phosphoinositides and is implicated in cellular processes such as membrane trafficking and remodeling of the actin cytoskeleton. Both ASAP1 and ASAP3 have recently been implicated in the development of human cancers. Abnormal activity of ArfGAPs may lead to the alterations characteristic of malignant cells, such as the high rate of proliferation and invasion. More specifically, ASAP3 has been found to be up-regulated in 80 % of the hepatocellular carcinomas examined. Initial biochemical characterization reveals that ASAP3 shows class-specific GAP activity on Arf proteins, preferring Arf5 over Arf1 and Arf6.Synonyms: ACAP4, ANK repeat and PH domain-containing protein 3, ASAP3, Arf-GAP with SH3 domain, CENTB6, Development and differentiation-enhancing factor-like 1, Protein up-regulated in liver cancer 1, UPLC1