FANCA
Reactivité: Humain
WB
Hôte: Lapin
Monoclonal
unconjugated
Indications d'application
ELISA: 1: 15,000 - 1: 60,000. Western blot: 1: 500 - 1: 3,000 (Expect a main band at approximately 163 kDa in sizecorresponding to FANCA by western blotting in the appropriate cell lysate or extract orhuman tissue). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
1.45 mg/mL (by UV absorbance at 280 nm)
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.
Stock
4 °C/-20 °C
Stockage commentaire
Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.
anticorps FA, anticorps FA-H, anticorps FA1, anticorps FAA, anticorps FACA, anticorps FAH, anticorps FANCH, anticorps AW208693, anticorps Fanconi anemia complementation group A, anticorps Fanconi anemia, complementation group A, anticorps FANCA, anticorps Fanca
Sujet
FANCA (also called Protein FACA or Fanconi anemia group A protein) is involved in DNA repair, perhaps specifically with post-replication repair or a cell cycle checkpoint function. FANCA may also be implicated in interstrand DNA cross-link repair and in the maintenance of normal chromosome stability. The Fanconi anemia complementation group (FANC) currently includes FANCA, FANCB, FANCC, FANCD1 (also called BRCA2), FANCD2, FANCE, FANCF, FANCG, and FANCL. The previously defined group FANCH is the same as FANCA. Fanconi anemia is a genetically heterogeneous recessive disorder characterized by cytogenetic instability, hypersensitivity to DNA crosslinking agents, increased chromosomal breakage, and defective DNA repair. The members of the Fanconi anemia complementation group do not share sequence similarity, they are related by their assembly into a common nuclear protein complex. This gene encodes the protein for complementation group A. Alternative splicing results in multiple transcript variants encoding different isoforms. Variant 1 (isoform a) encodes the longest transcript. Variant 2 (isoform b) contains an alternate exon, which results in an early stop codon, compared to variant 1. Isoform b has a shorter C-terminus when compared to isoform a. Mutations in this gene are the most common cause of Fanconi anemia.Synonyms: FAA, FACA, FANCH, Fanconi anemia group A protein