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H2AFV anticorps (pSer137)

H2AFV Reactivité: Drosophila melanogaster WB, EIA Hôte: Lapin Polyclonal unconjugated
N° du produit ABIN401328
  • Antigène Voir toutes H2AFV Anticorps
    H2AFV (H2A Histone Family, Member V (H2AFV))
    Épitope
    • 7
    • 2
    • 2
    • 1
    • 1
    AA 132-141, pSer137
    Reactivité
    • 13
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Drosophila melanogaster
    Hôte
    • 10
    • 5
    Lapin
    Clonalité
    • 10
    • 5
    Polyclonal
    Conjugué
    • 11
    • 2
    • 1
    • 1
    Cet anticorp H2AFV est non-conjugé
    Application
    • 8
    • 7
    • 4
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    Western Blotting (WB), Enzyme Immunoassay (EIA)
    Specificité
    This antibody is directed against the phosphorylated form of Drosophila H2AvD protein at the pSer137 residue. Reactivity with non-phosphorylated Drosophila H2AvD is minimal by ELISA. A BLAST analysis was used to suggest little to no cross reactivity with H2AvD proteins from other sources based on a comparison using the immunizing sequence. Expect a band approximately 14 kDa in size corresponding to phosphorylated H2AvD protein by western blotting in the appropriate Drosophila tissue or cell lysate or extract. Less than 0.2 % reactivity is observed against the non-phosphorylated form of the immunizing peptide. This antibody is phospho specific for pS137 of H2AvD protein.
    Purification
    Affinity Chromatography: The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide.
    Immunogène
    Synthetic peptide corresponding to amino acids 132-141 of Drosophila melanogaster (fruit fly) H2AvD protein.
    Isotype
    IgG
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    Discover our top product H2AFV Anticorps primaire
  • Indications d'application
    ELISA: 1/4,000-1/16,000. Western Blot: 1/400-1/1,600.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Concentration
    1.0 mg/mL (by UV absorbance at 280 nm)
    Buffer
    0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide as preservative
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
    Conseil sur la manipulation
    Avoid repeated freezing and thawing.
    Stock
    -20 °C
    Stockage commentaire
    Store the antibody (in aliquots) at-20 °C. This product is stable for several weeks at 2-0 °C as an undiluted liquid. Dilute only prior to immediate use.
  • Antigène
    H2AFV (H2A Histone Family, Member V (H2AFV))
    Autre désignation
    Histone H2A.v (H2AFV Produits)
    Synonymes
    anticorps H2A.Z-2, anticorps H2afv, anticorps H2AV, anticorps h2av, anticorps C530002L11Rik, anticorps H2a.z-2, anticorps H2av, anticorps H2A.F/Z, anticorps h2afv, anticorps h2afz, anticorps H2A histone family, member V, anticorps H2A histone family member V, anticorps H2A histone family member V S homeolog, anticorps H2A histone family, member Va, anticorps H2afv, anticorps LOC100226437, anticorps H2AFV, anticorps h2afv.S, anticorps h2afva
    Sujet
    Variant histones H2A are synthesized throughout the cell cycle and are very different from classical Sphase regulated H2A. H2AvD is vital for viability, but the exact function of variant histones H2A is not known. H2A is a core component of the nucleosome, an octamer containing two molecules each of H2A, H2B, H3 and H4. The octamer wraps approximately 146 bp of DNA. HsAvD is expressed both maternally and zygotically and is found in embryos through to adults (female only). The human homologue, H2AX, is phosphorylated by ATM protein kinase when double strand DNA breaks occur. In mouse, H2AX "knock out" mice have an increased incidence of cancer.Synonyms: CG5499, H2A.F/Z, H2AFV, H2AV, H2AvD, His2Av, His2AvD
    ID gène
    43229
    NCBI Accession
    NP_524519
    UniProt
    P08985
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