MYL6 anticorps (AA 12-27)
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- Antigène Voir toutes MYL6 Anticorps
- MYL6 (Myosin Light Chain 6, Alkali, Smooth Muscle and Non Muscle (MYL6))
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Épitope
- AA 12-27
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Reactivité
- Humain, Souris, Rat
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp MYL6 est non-conjugé
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Application
- Western Blotting (WB), Enzyme Immunoassay (EIA)
- Specificité
- This antibody is directed against the regulatory light chain of smooth and nonmuscle Myosin. The antibody detects both unphosphorylated and monophosphorylated forms of the protein. BLAST search analysis was used to determine that the smooth and non-muscle forms of myosin regulatory light chain have identical sequences. Cross reactivity is expected.
- Purification
- Immunoaffinity Chromatography.
- Immunogène
- Synthetic peptide corresponding to amino acids 12-27 of Human Myosin light chain protein.
- Isotype
- IgG
- Top Product
- Discover our top product MYL6 Anticorps primaire
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- Indications d'application
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Tested in ELISA (1/5000-1/20000) and Western Blot (1/500-1/2000). Although not tested, this antibody is likely functional in Immunohistochemistry andImmunoprecipitation (1/100).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 0.71 mg/mL (by UV absorbance at 280 nm)
- Buffer
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide as preservative.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
- Avoid repeated freezing and thawing.
- Stock
- 4 °C/-20 °C
- Stockage commentaire
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
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- Antigène
- MYL6 (Myosin Light Chain 6, Alkali, Smooth Muscle and Non Muscle (MYL6))
- Autre désignation
- MYL6 (MYL6 Produits)
- Synonymes
- anticorps ESMLC, anticorps LC17, anticorps LC17-GI, anticorps LC17-NM, anticorps LC17A, anticorps LC17B, anticorps MLC-3, anticorps MLC1SM, anticorps MLC3NM, anticorps MLC3SM, anticorps zgc:103624, anticorps MLC3nm, anticorps Myln, anticorps myosin light chain 6, anticorps myosin, light chain 6, alkali, smooth muscle and non-muscle, anticorps myosin light chain 6 L homeolog, anticorps myosin, light polypeptide 6, alkali, smooth muscle and non-muscle, anticorps MYL6, anticorps Myl6, anticorps myl6, anticorps myl6.L
- Sujet
- Myosin is the major component of thick muscle filaments, and is a long asymmetric molecule containing a globular head and a long tail. The molecule consists of two heavy chains each ~200,000 daltons, and four light chains each ~16,000 - 21,000 daltons. Activation of smooth and cardiac muscle primarily involves pathways that increase calcium and myosin phosphorylation resulting in contraction. Myosin light chain phosphatase acts to regulate muscle contraction by dephosphorylating activated myosin light chain. The selected peptide sequence used to generate the polyclonal antibody is located near the amino terminal end of the polypeptide corresponding to the smooth/non-muscle form of myosin regulatory light chain found in cardiac myocytes in addition to smooth and non-muscle cells. This sequence differs from that of the sarcomeric/cardiac form of myosin regulatory light chain that has a different sequence around the phosphorylation site. Human, mouse and rat have almost identical sequences.Synonyms: 17 kDa myosin light chain, LC17, MLC-3, Myosin light chain 3, Myosin light chain alkali 3, Myosin light polypeptide 6, Smooth muscle and nonmuscle myosin light chain alkali 6
- ID gène
- 4637
- NCBI Accession
- NP_066299
- UniProt
- P60660
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