Species reactivity (expected):Mouse, Rat, Canine, Macaque, Chimpanzee, Opossum, Zebrafish, Green pufferfish, Platypus. Species reactivity (tested):Human.
Purification
Affinity chromatography
Immunogène
Purified peptide corresponding to amino acids near the N-terminal end of human PTEN-P1 protein
ELISA: 1/160,000. Western Blot: 1/500 to 1/2,000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
0.73 mg/mL (by UV absorbance at 280 nm)
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Avoid repeated freezing and thawing.
Stock
-20 °C
Antigène
PTENP1
(Phosphatase and Tensin Homolog Pseudogene 1 (PTENP1))
This gene (PTENP1) is a highly homologous pseudogene of PTEN. PTEN was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. PTEN is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tension like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway.Synonyms: PTEN-rs, PTEN2, PTH2, Phosphatase and tensin homolog 2, Phosphatase and tensin homolog pseudogene 1, psiPTEN