SAE1 anticorps (Ser185)
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- Antigène Voir toutes SAE1 Anticorps
- SAE1 (SUMO1 Activating Enzyme Subunit 1 (SAE1))
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Épitope
- Ser185
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Reactivité
- Humain, Souris, Rat, Boeuf (Vache), Chien, Chimpanzé
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp SAE1 est non-conjugé
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Application
- Western Blotting (WB), Enzyme Immunoassay (EIA)
- Specificité
- This antibody is directed against SUMO Activating Enzyme E1 protein.
- Réactivité croisée (Details)
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Species reactivity (expected):Bovine, Canine, Chimpanzee, Mouse, Rat.
Species reactivity (tested):Human. - Purification
- Affinity chromatography
- Immunogène
- Synthetic peptide corresponding to a region surrounding S185 of the human SUMO Activating Enzyme E1 protein
- Isotype
- IgG
- Top Product
- Discover our top product SAE1 Anticorps primaire
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- Indications d'application
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ELISA: 1/100,000 - 1/125,000. Western Blot: 1/750 - 1/1,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.71 mg/mL (by UV absorbance at 280 nm)
- Buffer
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
- Avoid repeated freezing and thawing.
- Stock
- -20 °C
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- Antigène
- SAE1 (SUMO1 Activating Enzyme Subunit 1 (SAE1))
- Autre désignation
- SAE1 / AOS1 (SAE1 Produits)
- Synonymes
- anticorps AOS1, anticorps HSPC140, anticorps SUA1, anticorps UBLE1A, anticorps 2400010M20Rik, anticorps 2610044L12Rik, anticorps AL033372, anticorps AW743391, anticorps D7Ertd177e, anticorps Sua1, anticorps Uble1a, anticorps Aos1p, anticorps Sua1p, anticorps aos, anticorps sae2a, anticorps uble1a, anticorps wu:fa28b04, anticorps zgc:86633, anticorps SUMO1 activating enzyme subunit 1, anticorps SUMO1 activating enzyme subunit 1 L homeolog, anticorps SAE1, anticorps Sae1, anticorps sae1.L, anticorps sae1
- Sujet
- SUMO E1 activating enzyme (also called Ubiquitin-like 1 activating enzyme E1A, UBLE1A, AOS1, SAE1, and SUA1) with SAE2 (also known as UBA2) forms a heterodimeric (SAE1/SAE2) enzyme that activates the ubiquitinlike SUMO proteins (SUMO stands for Small Ubiquitin-like MOdifier.) The SAE1 (SUMO Activating Enzyme 1) subunit resembles the N-terminal half of yeast UBA1, the SAE2 (also called Uba2) subunit corresponds to the C-terminal part of yeast UBA1 and contains the active site cysteine. In the SUMO activation step, SAE1/SAE2 uses ATP to adenylate the C-terminal glycine of SUMO-1 (the first of the three different mammalian SUMO proteins) then forms a high-energy thioester bond between the C-terminal glycine and the active site cysteine in SAE2 (Uba2). In the conjugation step, the SUMO moiety is transferred from SAE1/SAE2 to the active site cysteine (Cys 93) of the SUMO conjugating enzyme (SUMO E2, Ubc9) forming a SUMO-E2 thioester complex.Synonyms: SUA1, SUMO-activating enzyme subunit 1, UBLE1A, Ubiquitin-like 1-activating enzyme E1A
- ID gène
- 10055
- NCBI Accession
- NP_005491
- UniProt
- Q9UBE0
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