GATA3 anticorps (N-Term)
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- Antigène Voir toutes GATA3 Anticorps
- GATA3 (GATA Binding Protein 3 (GATA3))
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Épitope
- N-Term
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Reactivité
- Humain, Souris, Rat, Boeuf (Vache), Porc, Chien
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Hôte
- Chèvre
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp GATA3 est non-conjugé
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Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
- Séquence
- EVTADQPRWV SHH-C
- Specificité
- This antibody detects GATA3 at N-term. It is expected to recognize both reported isoforms (NP_001002295.1 and NP_002042.1).
- Réactivité croisée (Details)
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Species reactivity (expected):Mouse, Rat, Dog, Pig, Cow.
Species reactivity (tested):Human. - Purification
- Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
- Immunogène
- Peptide from the N Terminus of the protein sequence according to NP_001002295.1, NP_002042.1 . Genename: GATA3
- Top Product
- Discover our top product GATA3 Anticorps primaire
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- Indications d'application
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Peptide ELISA: Antibody detection limit dilution 1: 32000. Western blot: 1-3 μg/mL. Approx 50 kDa band observed in lysates of cell line MOLT4(calculated MW of 48.0 according to NP_001002295.1 and 47.0 kDa according toNP_002042.1). Immunohistochemistry: In paraffin embedded Human Tonsil, stains nuclei of some cells inthe interfollicular area and in germinal centres. Recommended concentration, 3 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 0.5 mg/mL
- Buffer
- Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
- Avoid repeated freezing and thawing.
- Stock
- 4 °C/-20 °C
- Stockage commentaire
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
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Mycoleptodonoides aitchisonii suppresses asthma via Th2 and Th1 cell regulation in an ovalbumin‑induced asthma mouse model." dans: Molecular medicine reports, Vol. 17, Issue 1, pp. 11-20, (2018) (PubMed).
: "Erythronium japonicum attenuates histopathological lung abnormalities in a mouse model of ovalbumin-induced asthma." dans: International journal of molecular medicine, Vol. 37, Issue 5, pp. 1221-8, (2016) (PubMed).
: "Bacillus subtilis KCTC 11782BP-Produced Alginate Oligosaccharide Effectively Suppresses Asthma via T-Helper Cell Type 2-Related Cytokines." dans: PLoS ONE, Vol. 10, Issue 2, pp. e0117524, (2015) (PubMed).
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Mycoleptodonoides aitchisonii suppresses asthma via Th2 and Th1 cell regulation in an ovalbumin‑induced asthma mouse model." dans: Molecular medicine reports, Vol. 17, Issue 1, pp. 11-20, (2018) (PubMed).
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- Antigène
- GATA3 (GATA Binding Protein 3 (GATA3))
- Autre désignation
- GATA3 (GATA3 Produits)
- Synonymes
- anticorps HDR, anticorps HDRS, anticorps Gata-3, anticorps jal, anticorps cb550, anticorps gta3, anticorps GATA-3, anticorps XGATA3, anticorps xgata-3, anticorps hdr, anticorps xGATA-3, anticorps GATA3, anticorps GATA transcription factor 3, anticorps T4L20.260, anticorps T4L20_260, anticorps gata3, anticorps ZH-DR, anticorps zgc:103531, anticorps GATA binding protein 3, anticorps GATA binding protein 3 S homeolog, anticorps GATA transcription factor 3, anticorps amidase, anticorps putative amidase, anticorps GATA-binding protein 3, anticorps hematopoietic death receptor, anticorps GATA3, anticorps Gata3, anticorps gata3, anticorps gata3.S, anticorps gatA3, anticorps hdr
- Sujet
- The genes for all 4 subunits of the T-cell antigen receptor (alpha, beta, gamma and delta) are controlled by distinct enhancers and their enhancer-binding proteins. Marine and Winoto (1991) identified a common TCR regulatory element by demonstrating binding of the enhancer-binding protein GATA3 to the enhancer elements of all 4 TCR genes. GATA3 had been shown in the chicken to be an enhancer-binding protein containing a zinc finger domain. GATA3 mRNA was demonstrated by Northern blot analysis in T cells but not in B cells or macrophages. GATA3 is abundantly expressed in the T-lymphocyte lineage and is thought to participate in T-cell receptor gene activation through binding to enhancers. Labastie et al. (1994) cloned the human gene and the 5-prime end of the mouse gene. The human gene comprises 6 exons distributed over 17 kb of DNA. Its 2 zinc fingers are encoded by 2 separate exons highly conserved with those of GATA1, but no other structural homologies between the 2 genes could be found.Synonyms: GATA-3, GATA-binding factor 3, Trans-acting T-cell-specific transcription factor GATA-3
- ID gène
- 2625
- NCBI Accession
- NP_001002295
- UniProt
- P23771
- Pathways
- Hormone Transport, Regulation of Hormone Metabolic Process, Tube Formation
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