Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Specificité
This antibody detects endogenous levels of MARCKS only when phosphorylated at Threonine 170.
Purification
Immunoaffinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Immunogène
The antiserum was produced against synthesized phosphopeptide derived from human MARCKS around the phosphorylation site of Serine 170 (G-F-Sp-F-K).
MARCKS
Reactivité: Rat
ELISA, IF (cc), IF (p), IHC (p), IHC (fro)
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
Western blot: 1/500approx. 1/1000. Immunohistochemistry on Paraffin Sections: 1/50approx. 1/100. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
1.0 mg/mL
Buffer
PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Avoid repeated freezing and thawing.
Stock
-20 °C
Stockage commentaire
Store the antibody (in aliquots) at-20 °C.
Antigène
MARCKS
(Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS))
anticorps 80K-L, anticorps MACS, anticorps PKCSL, anticorps PRKCSL, anticorps KINC, anticorps Macs, anticorps marcks, anticorps MGC89127, anticorps MARCKS, anticorps macs, anticorps wu:fb55c06, anticorps wu:fb92d04, anticorps wu:fc19b04, anticorps wu:fc44a10, anticorps zgc:110235, anticorps fc34g03, anticorps fc38c06, anticorps wu:fc34g03, anticorps wu:fc38c06, anticorps zgc:109978, anticorps myristoylated alanine rich protein kinase C substrate, anticorps myristoylated alanine-rich protein kinase C substrate, anticorps methyl binding domain106, anticorps myristoylated alanine-rich protein kinase C substrate b, anticorps myristoylated alanine-rich protein kinase C substrate S homeolog, anticorps myristoylated alanine-rich protein kinase C substrate a, anticorps MARCKS, anticorps Marcks, anticorps marcks, anticorps mbd106, anticorps marcksb, anticorps marcks.S, anticorps marcksa
Sujet
MARCKS, (Myristoylated Alanine-Rich C Kinase Substrate), is a member of a family of calmodulin binding proteins and is a major substrate for phosphorylation by protein kinase C (PKC). The phosphorylation of Ser152/156 can be used as a measure of PKC activation. Phosphorylation of Ser152/156 modulates the binding of MARCKS to calmodulin.Synonyms: MACS, Myristoylated alanine-rich C-kinase substrate, PRKCSL