Applications: FC - Quality tested , IHC-FS - Reported in literature , ICC - Reported in literature , IP - Reported in literature
Working Dilutions: Flow Cytometry Purified (UNLB) antibody 1 g/106 cells FITC and BIOT conjugates 1 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L
Volume d'échantillon
1 mL
Restrictions
For Research Use only
Concentration
0.5 mg/mL
Buffer
0.5 mg of purified immunoglobulin in 1.0 mL of borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added
Agent conservateur
Without preservative
Conseil sur la manipulation
Each reagent is stable for the period shown on the bottle label if stored as directed.
CD1 molecules, like MHC I and II, play an equally important role in the immune system by presenting lipid, glycolipid and lipopeptide antigen to T and NKT cells 1. Chicken CD1 gene is located in the chicken MHC B locus and has an important implication for the primordial MHC gene evolution 2. Two proteins have been identified: CD1.1 and CD1.2. Analysis of RNA from blood cells showed that both genes are expressed in Bu-1+ cells (B cells and some macrophages) and CD8a+ cells (some T and NK cells). CD1.2 is expressed in TCR1 + cells (γ δ cells) but is nearly undetectable in TCR2+ and TCR3+ cells (α and β T cells) 1.