BLNK
Reactivité: Humain, Souris, Rat
WB, ELISA, IHC
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
ELISA: 1/1,000. Western Blot: 1/100approx. 1/500. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Format
Liquid
Concentration
0.25 mg/mL
Buffer
PBS with 0.09 % (W/V) Sodium Azide as preservative
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Avoid repeated freezing and thawing.
Stock
4 °C/-20 °C
Stockage commentaire
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
BLNK (B cell linker protein) is an adaptor molecule that plays key role in B cell activation initiated by cross-linking the B cell receptor (BCR). This activation leads to an increase in the tyrosine phosphorylation of a number of proteins due to activation of nonreceptor protein tyrosine kinases (PTK) including Syk, which is responsible for the tyrosine phosphorylation of BLNK. BLNK is cytosolic protein identified as two phosphoproteins migrating at 68 and 70 kDa in SDS/PAGE (alternatively spliced forms of human BLNK). Mapping of the tyrosine residues in BLNK reveal three tyrosines that bind PLC-gamma2, and two tyrosines that bind the Tec family kinase Btk, the Vav nucleotide exchange factor and the adaptor Nck.Synonyms: B-cell adapter containing a SH2 domain protein, B-cell adapter containing a Src homology 2 domain protein, BASH, Cytoplasmic adapter protein, SLP-65, SLP65, Src homology 2 domain-containing leukocyte protein of 65 kDa