ELISA: 1/1,000. Western blotting: 1/50 - 1/100. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Format
Liquid
Concentration
0.25 mg/mL
Buffer
PBS with 0.09 % (W/V) sodium azide
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Avoid repeated freezing and thawing.
Stock
4 °C/-20 °C
Stockage commentaire
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Antigène
CYP8B1
(Cytochrome P450, Family 8, Subfamily B, Polypeptide 1 (CYP8B1))
anticorps MGC64456, anticorps CYP8B, anticorps CP8B, anticorps CYP12, anticorps cytochrome P450 family 8 subfamily B member 1, anticorps cytochrome P450 family 8 subfamily B member 1 S homeolog, anticorps cytochrome P450, family 8, subfamily B, polypeptide 1, anticorps cytochrome P450, family 8, subfamily b, polypeptide 1, anticorps Cyp8b1, anticorps cyp8b1.S, anticorps CYP8B1
Sujet
CYP8B1 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum membrane protein catalyzes the conversion of 7 alpha-hydroxy-4-cholesten-3-one into 7-alpha,12-alpha-dihydroxy-4-cholesten-3-one. The balance between these two steroids determines the relative amounts of cholic acid and chenodeoxycholic acid both of which are secreted in the bile and affect the solubility of cholesterol. This gene is unique among the cytochrome P450 genes in that it is intronless.Synonyms: 7-alpha-hydroxy-4-cholesten-3-one 12-alpha-hydroxylase, 7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase, CYP12, CYPVIIIB1, Cytochrome P450 8B1, Sterol 12-alpha-hydroxylase