CYB5R3 anticorps (C-Term)

N° du produit ABIN453978

Cité dans 3 publications. Cet anticorps anti-CYB5R3 Polyclonal Chèvre (ABIN453978) détecte spécifiquement CYB5R3 dans WB et EIA. L’anticorps est réactif avec des échantillons de Rat, Souris, Chien et Boeuf (Vache).

Aperçu rapide pour CYB5R3 anticorps (C-Term) (ABIN453978)

Antigène: CYB5R3 (Cytochrome B5 Reductase 3 (CYB5R3))

Reactivité: Rat, Souris, Chien, Boeuf (Vache)

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp CYB5R3 est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Détail du produit anti-CYB5R3 anticorps

Épitope: C-Term

Specificité: This antibody recognizes CYB5R3.

Réactivité croisée (Details): Species reactivity (expected):Rat, Canine (Dog) and Cow.
Species reactivity (tested):Mouse.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogène: Peptide with sequence PNLERVGHPKERC, from the C-Terminus of the protein sequence according to NP_084063.1.

Information d'application

Indications d'application: Peptide ELISA: Detection limit dilution 1/32000. Western blot: 0.1-1 μg/mL. Approx 35 kDa band observed in Mouse Liver lysates as well as inMouse and Rat brain lysates (calculated MW of 34.5 kDa according to Mouse NP_084063.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Species Reactivity: Tested: Mouse. Expected from sequence similarity: Rat, Canine (Dog) and Cow.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Références pour anticorps anti-CYB5R3 (ABIN453978)

Reed, Jarvis, Phillips, Arlt: "Enhanced DNA adduct formation by benzo[a]pyrene in human liver cells lacking cytochrome P450 oxidoreductase." dans: Mutation research, Vol. 852, pp. 503162, (2020) (PubMed).

Reed, Indra, Mrizova, Moserova, Schmeiser, Wolf, Henderson, Stiborova, Phillips, Arlt: "Application of hepatic cytochrome b5/P450 reductase null (HBRN) mice to study the role of cytochrome b5 in the cytochrome P450-mediated bioactivation of the anticancer drug ellipticine." dans: Toxicology and applied pharmacology, Vol. 366, pp. 64-74, (2019) (PubMed).

Reed, Mrizova, Barta, Indra, Moserova, Kopka, Schmeiser, Wolf, Henderson, Stiborova, Phillips, Arlt: "Cytochrome b 5 impacts on cytochrome P450-mediated metabolism of benzo[a]pyrene and its DNA adduct formation: studies in hepatic cytochrome b 5 /P450 reductase null (HBRN) mice." dans: Archives of toxicology, Vol. 92, Issue 4, pp. 1625-1638, (2019) (PubMed).

Détails sur CYB5R3

Antigène: CYB5R3 (Cytochrome B5 Reductase 3 (CYB5R3))

Autre désignation: CYB5R3

Sujet: CYB5R3 belongs to the flavoprotein pyridine nucleotide cytochrome reductase family and contains 1 FAD-binding FR-type domain. There are two isoforms, NADH-cytochrome b5 reductase 3 membrane bound form and NADH-cytochrome b5 reductase 3 soluble form. Its functions include desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction. Defects in CYB5R3 are the cause of hereditary methemoglobinemia (HM). There are three forms of this disease: type 1 (HM1) in which the enzyme is only deficient in erythrocytes with a mild cyanosis, type 2 (HM2), in which the enzyme is completely deficient, type 3 (HM3) where the deficiency is seen in all blood cells. Type 2 is a severe form accompanied with mental retardation and neurological impairment.Synonyms: B5R, DIA1, Diaphorase-1, NADH-cytochrome b5 reductase 3

ID gène: 1727

NCBI Accession: NP_000389

UniProt: P00387

Pathways: SARS-CoV-2 Protein Interactome