RNF34 anticorps (N-Term)

N° du produit ABIN453991

Cet anticorps Chèvre Polyclonal détecte spécifiquement RNF34 dans IHC (p) et EIA. Il présente une réactivité avec des échantillons de Humain, Souris, Rat, Boeuf (Vache) et Chien.

Aperçu rapide pour RNF34 anticorps (N-Term) (ABIN453991)

Antigène: RNF34 (Ring Finger Protein 34 (RNF34))

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Chien

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp RNF34 est non-conjugé

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Détail du produit anti-RNF34 anticorps

Épitope: N-Term

Specificité: This antibody is expected to recognize both reported isoforms of Human RNF34 (NP_919247.1 and NP_079402.2).

Réactivité croisée (Details): Species reactivity (expected):Mouse, Rat, Canine (Dog) and Cow.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogène: Peptide with sequence KAGATSMWASCC, from the N Terminus of the protein sequence according to NP_919247.1, NP_079402.2. Genename: RNF34

Information d'application

Indications d'application: Peptide ELISA: Limit Dilution: 1/32000. Immunohistochemistry: 3-5 μg/mL. In paraffin embedded Human Kidney shows strongstaining of cytoplasm in DCT.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Détails sur RNF34

Antigène: RNF34 (Ring Finger Protein 34 (RNF34))

Autre désignation: RFI / RNF34

Sujet: RNF34, also known as RING finger protein 34 is a novel modulator of NF-kappaB activation. RNF34 possesses E3 ubiquitin protein ligase activity and has been shown to regulate the levels of CASP8 and CASP10 by targeting them for proteasomal degradation. RNF34 protects cells against apoptosis induced by TNF and may bind phosphatidylinositol phosphates. RNF34 is localized to both the nucleus and the cytoplasm. In the nucleus, a distinct speckled pattern is observed by immunofluorescence microscopy. In the cytoplasm, the protein is associated with membranes. RNF34 is expressed ubiquitously and can be detected in heart, brain, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, ovary, colon and leukocytes. Multiple transcript variants have been reported for this protein.Synonyms: CARP-1, CARP1, Caspases-8 and -10-associated RING finger protein 1, E3 ubiquitin-protein ligase RNF34, FYVE-RING finger protein Momo, Human RING finger homologous to inhibitor of apoptosis protein, RIFF

ID gène: 80196

NCBI Accession: NP_079402

UniProt: Q969K3