L-Selectin anticorps (FITC)

N° du produit ABIN457338

Détail du produit anti-L-Selectin anticorps (FITC)

Antigène: L-Selectin (SELL) (Selectin L (SELL))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp L-Selectin est conjugé à/à la FITC

Application: Flow Cytometry (FACS)

Specificité: The mouse monoclonal antibody DREG56 recognizes an extracellular epitope of CD62L / L-selectin, a 65-76 kDa cell surface protein, expressed by neutrophils, monocytes, and subsets of T, B, and NK cells, that interacts with specific carbohydrates exposed on activated endothelial cells.

Réactivité croisée (Details): Human

Purification: Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogène: PMA-activated human peripheral blood leukocytes

Clone: DREG56

Isotype: IgG1

Information d'application

Indications d'application: Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.

Commentaires:

The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use. No reconstitution is necessary.

Restrictions: For Research Use only

Stockage

Reconstitution: No reconstitution is necessary.

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze.
Avoid prolonged exposure to light.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Références pour anticorps anti-L-Selectin (FITC) (ABIN457338)

Tu, Mao, Zheng, Liu, Chiu, Qin, Chan, Lam, Guan, Zhang, Guan, Yuen, Peiris, Lau: "Cytotoxic T lymphocytes established by seasonal human influenza cross-react against 2009 pandemic H1N1 influenza virus." dans: Journal of virology, Vol. 84, Issue 13, pp. 6527-35, (2010) (PubMed).

Killock, Parsons, Zarrouk, Ameer-Beg, Ridley, Haskard, Zvelebil, Ivetic: "In Vitro and in Vivo Characterization of Molecular Interactions between Calmodulin, Ezrin/Radixin/Moesin, and L-selectin." dans: The Journal of biological chemistry, Vol. 284, Issue 13, pp. 8833-45, (2009) (PubMed).

Xu, Chen, Shang, Cui, Luo, Chen, Ba, Zeng: "Critical role of Lck in L-selectin signaling induced by sulfatides engagement." dans: Journal of leukocyte biology, Vol. 84, Issue 4, pp. 1192-201, (2008) (PubMed).

Jutila, Kurk, Jackiw, Knibbs, Stoolman: "L-selectin serves as an E-selectin ligand on cultured human T lymphoblasts." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 169, Issue 4, pp. 1768-73, (2002) (PubMed).

Abraham, Ahmed, Sabater, Lauredo, Botvinnikova, Bjercke, Hu, Revelle, Kogan, Scott, Dixon, Yeh, Beck: "Selectin blockade prevents antigen-induced late bronchial responses and airway hyperresponsiveness in allergic sheep." dans: American journal of respiratory and critical care medicine, Vol. 159, Issue 4 Pt 1, pp. 1205-14, (1999) (PubMed).

Kishimoto, Warnock, Jutila, Butcher, Lane, Anderson, Smith: "Antibodies against human neutrophil LECAM-1 (LAM-1/Leu-8/DREG-56 antigen) and endothelial cell ELAM-1 inhibit a common CD18-independent adhesion pathway in vitro." dans: Blood, Vol. 78, Issue 3, pp. 805-11, (1991) (PubMed).

Kishimoto, Jutila, Butcher: "Identification of a human peripheral lymph node homing receptor: a rapidly down-regulated adhesion molecule." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 87, Issue 6, pp. 2244-8, (1990) (PubMed).

Détails sur L-Selectin

Antigène: L-Selectin (SELL) (Selectin L (SELL))

Autre désignation: CD62L (SELL Produits)

Synonymes: anticorps CD62L, anticorps LAM1, anticorps LECAM1, anticorps LEU8, anticorps LNHR, anticorps LSEL, anticorps LYAM1, anticorps PLNHR, anticorps TQ1, anticorps A.11, anticorps L-selectin, anticorps LECAM-1, anticorps SELE, anticorps AI528707, anticorps Lnhr, anticorps Ly-22, anticorps Ly-m22, anticorps Lyam-1, anticorps Lyam1, anticorps selectin L, anticorps selectin, lymphocyte, anticorps SELL, anticorps Sell

Sujet: Selectin L,CD62L (L-selectin) is an adhesion glycoprotein that is constitutively expressed on the cell surface of leukocytes and mediates their homing to inflammatory sites and peripheral lymph nodes by enabling rolling along the venular wall. CD62L is also involved in activation-induced neutrophil aggregation. Activation-dependent CD62L shedding, however, counteracts neutrophil rolling. CD62L has also signaling roles including enhance of chemokine receptor expression. Similarly to CD62P, the major ligand of CD62L is PSGL-1 (P-selectin glycoprotein ligand-1).,L-selectin, LSEL, SELL, LAM-1, LECAM1, LEU8, LNHR, LYAM1, PLNHR

ID gène: 6402

UniProt: P14151