ITGAL anticorps (FITC)
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- Antigène Voir toutes ITGAL Anticorps
- ITGAL (Integrin alpha-L (ITGAL))
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Reactivité
- Souris
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Hôte
- Rat
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp ITGAL est conjugé à/à la FITC
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Application
- Flow Cytometry (FACS)
- Specificité
- The rat monoclonal antibody M17/4 reacts with an extracellular epitope of CD11a (alpha-subunit of murine LFA-1), a 180 kDa type I transmembrane glycoprotein expressed on B and T lymphocytes, monocytes, macrophages, neutrophils, basophils and eosinophils.
- Réactivité croisée (Details)
- Mouse
- Purification
- Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
- Immunogène
- C57BL/6 mouse splenic secondary cytotoxic T lymphocytes
- Clone
- M17-4
- Isotype
- IgG2a
- Top Product
- Discover our top product ITGAL Anticorps primaire
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- Indications d'application
- Flow cytometry: Recommended dilution: 1-2 μg/mL.
- Commentaires
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The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC.
- Restrictions
- For Research Use only
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- Concentration
- 0.5 mg/mL
- Buffer
- Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
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Do not freeze.
Avoid prolonged exposure to light. - Stock
- 4 °C
- Stockage commentaire
- Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
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Soluble MHC-peptide complexes containing long rigid linkers abolish CTL-mediated cytotoxicity." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 176, Issue 6, pp. 3356-65, (2006) (PubMed).
: "The beta2 integrin CD11c distinguishes a subset of cytotoxic pulmonary T cells with potent antiviral effects in vitro and in vivo." dans: Respiratory research, Vol. 6, pp. 70, (2005) (PubMed).
: "Adhesion molecule-dependent mechanisms regulate the rate of macrophage clearance during the resolution of peritoneal inflammation." dans: The Journal of experimental medicine, Vol. 196, Issue 11, pp. 1515-21, (2002) (PubMed).
: "In situ demonstration of intraepithelial lymphocyte adhesion to villus microvessels of the small intestine." dans: International immunology, Vol. 13, Issue 9, pp. 1165-74, (2001) (PubMed).
: "Synergistic mobilization of hemopoietic progenitor cells using concurrent beta1 and beta2 integrin blockade or beta2-deficient mice." dans: Blood, Vol. 97, Issue 5, pp. 1282-8, (2001) (PubMed).
: "The accessory function of murine intercellular adhesion molecule-1 in T lymphocyte activation. Contributions of adhesion and co-activation." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 146, Issue 6, pp. 1773-82, (1991) (PubMed).
: "Antigens involved in mouse cytolytic T-lymphocyte (CTL)-mediated killing: functional screening and topographic relationship." dans: Cellular immunology, Vol. 73, Issue 1, pp. 1-11, (1983) (PubMed).
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Soluble MHC-peptide complexes containing long rigid linkers abolish CTL-mediated cytotoxicity." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 176, Issue 6, pp. 3356-65, (2006) (PubMed).
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- Antigène
- ITGAL (Integrin alpha-L (ITGAL))
- Autre désignation
- CD11a (ITGAL Produits)
- Synonymes
- anticorps CD11A, anticorps LFA-1, anticorps LFA1A, anticorps (p180), anticorps Cd11a, anticorps LFA-1A, anticorps Ly-15, anticorps Ly-21, anticorps CD11a, anticorps ITGAL, anticorps integrin subunit alpha L, anticorps integrin alpha L, anticorps ITGAL, anticorps Itgal
- Sujet
- Integrin subunit alpha L,CD11a (LFA-1 alpha) together with CD18 constitute leukocyte function-associated antigen 1 (LFA-1), the alphaLbeta2 integrin. CD11a is implicated in activation of LFA-1 complex. LFA-1 is expressed on the plasma membrane of leukocytes in a low-affinity conformation. Cell stimulation by chemokines or other signals leads to induction the high-affinity conformation, which supports tight binding of LFA-1 to its ligands, the intercellular adhesion molecules ICAM-1, -2, -3. LFA-1 is thus involved in interaction of various immune cells and in their tissue-specific settlement, but participates also in control of cell differentiation and proliferation and of T-cell effector functions. Blocking of LFA-1 function by specific antibodies or small molecules has become an important therapeutic approach in treatment of multiple inflammatory diseases. For example, humanized anti-LFA-1 antibody Efalizumab (Raptiva) is being used to interfere with T cell migration to sites of inflammation, binding of cholesterol-lowering drug simvastatin to CD11a allosteric site leads to immunomodulation and increase in lymphocytic cholinergic activity.,LFA-1, LFA1A, ITGAL
- ID gène
- 16408
- UniProt
- E9Q5M7
- Pathways
- Activated T Cell Proliferation, Integrin Complex
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