MHC Class II anticorps (FITC)
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- Antigène Voir toutes MHC Class II (MHC2) Anticorps
- MHC Class II (MHC2) (Major Histocompatibility Complex Class II Antigen (MHC2))
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Reactivité
- Souris
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Hôte
- Rat
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp MHC Class II est conjugé à/à la FITC
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Application
- Flow Cytometry (FACS)
- Specificité
- The rat monoclonal antibody M5/114 reacts with murine MHC class II glycoproteins. It recognizes a shared extracellular determinant on I-Ab, I-Ad, I-Aq, and I-Ed, I-Ek alloantigens, but it does not react with I-Af, I-Ak, I-As. This antibody can inhibit I-A-restricted T cell responses of the H-2b, H-2d, H-2q, H-2u but not H-2f, H-2k, H-2s haplotypes.
- Réactivité croisée (Details)
- Mouse
- Purification
- Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
- Immunogène
- Activated C57BL/6 mouse spleen cells
- Clone
- M5-114
- Isotype
- IgG2b
- Top Product
- Discover our top product MHC2 Anticorps primaire
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- Indications d'application
- Flow cytometry: Recommended dilution: 2-3 μg/mL.
- Commentaires
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The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC.
- Restrictions
- For Research Use only
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- Concentration
- 0.5 mg/mL
- Buffer
- Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
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Do not freeze.
Avoid prolonged exposure to light. - Stock
- 4 °C
- Stockage commentaire
- Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
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CD83 influences cell-surface MHC class II expression on B cells and other antigen-presenting cells." dans: International immunology, Vol. 19, Issue 8, pp. 977-92, (2007) (PubMed).
: "Cathepsin S controls MHC class II-mediated antigen presentation by epithelial cells in vivo." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 174, Issue 3, pp. 1205-12, (2005) (PubMed).
: "MHC Class II-mediated apoptosis by a nonpolymorphic MHC Class II peptide proceeds by activation of protein kinase C." dans: Journal of the American Society of Nephrology : JASN, Vol. 16, Issue 12, pp. 3661-8, (2005) (PubMed).
: "Reorganization of multivesicular bodies regulates MHC class II antigen presentation by dendritic cells." dans: The Journal of cell biology, Vol. 155, Issue 1, pp. 53-63, (2001) (PubMed).
: "Residual MHC class II expression on mature dendritic cells and activated B cells in RFX5-deficient mice." dans: Immunity, Vol. 8, Issue 2, pp. 143-55, (1998) (PubMed).
: "Intracellular Leishmania amazonensis amastigotes internalize and degrade MHC class II molecules of their host cells." dans: Journal of cell science, Vol. 108 ( Pt 10), pp. 3219-31, (1995) (PubMed).
: "Mice lacking the MHC class II-associated invariant chain." dans: Cell, Vol. 72, Issue 4, pp. 635-48, (1993) (PubMed).
: "A shared alloantigenic determinant on Ia antigens encoded by the I-A and I-E subregions: evidence for I region gene duplication." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 127, Issue 6, pp. 2488-95, (1982) (PubMed).
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CD83 influences cell-surface MHC class II expression on B cells and other antigen-presenting cells." dans: International immunology, Vol. 19, Issue 8, pp. 977-92, (2007) (PubMed).
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- Antigène
- MHC Class II (MHC2) (Major Histocompatibility Complex Class II Antigen (MHC2))
- Autre désignation
- MHC Class II (MHC2 Produits)
- Synonymes
- anticorps H-2, anticorps MHC-II, anticorps histocompatibility-2, MHC, anticorps H2
- Sujet
- MHC (major histocompatibility complex) class II molecules are transmembrane glycoproteins expressed on the surface of professional antigen-presenting cells, such as macrophages, dendritic cells and B cells. Before their exposition on the cell surface, the MHC class II molecules react with endocytosed exogenous antigens, which are then presented to the T cells. The antigen-binding grove between MHC class II alpha and beta chain is open at both ends and is 15-24 amino acid residues long.
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