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MHC Class II anticorps (FITC)

MHC2 Reactivité: Souris FACS Hôte: Rat Monoclonal M5-114 FITC
N° du produit ABIN457349
  • Antigène Voir toutes MHC Class II (MHC2) Anticorps
    MHC Class II (MHC2) (Major Histocompatibility Complex Class II Antigen (MHC2))
    Reactivité
    • 48
    • 45
    • 13
    • 9
    • 9
    • 6
    • 4
    • 2
    • 2
    • 1
    • 1
    Souris
    Hôte
    • 45
    • 27
    • 23
    Rat
    Clonalité
    • 75
    • 19
    • 1
    Monoclonal
    Conjugué
    • 29
    • 14
    • 10
    • 10
    • 5
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp MHC Class II est conjugé à/à la FITC
    Application
    • 67
    • 40
    • 22
    • 20
    • 18
    • 13
    • 13
    • 9
    • 5
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS)
    Specificité
    The rat monoclonal antibody M5/114 reacts with murine MHC class II glycoproteins. It recognizes a shared extracellular determinant on I-Ab, I-Ad, I-Aq, and I-Ed, I-Ek alloantigens, but it does not react with I-Af, I-Ak, I-As. This antibody can inhibit I-A-restricted T cell responses of the H-2b, H-2d, H-2q, H-2u but not H-2f, H-2k, H-2s haplotypes.
    Réactivité croisée (Details)
    Mouse
    Purification
    Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
    Immunogène
    Activated C57BL/6 mouse spleen cells
    Clone
    M5-114
    Isotype
    IgG2b
    Top Product
    Discover our top product MHC2 Anticorps primaire
  • Indications d'application
    Flow cytometry: Recommended dilution: 2-3 μg/mL.
    Commentaires

    The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC.

    Restrictions
    For Research Use only
  • Concentration
    0.5 mg/mL
    Buffer
    Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Conseil sur la manipulation
    Do not freeze.
    Avoid prolonged exposure to light.
    Stock
    4 °C
    Stockage commentaire
    Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
  • Kuwano, Prazma, Yazawa, Watanabe, Ishiura, Kumanogoh, Okochi, Tamaki, Fujimoto, Tedder: "CD83 influences cell-surface MHC class II expression on B cells and other antigen-presenting cells." dans: International immunology, Vol. 19, Issue 8, pp. 977-92, (2007) (PubMed).

    Beers, Burich, Kleijmeer, Griffith, Wong, Rudensky: "Cathepsin S controls MHC class II-mediated antigen presentation by epithelial cells in vivo." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 174, Issue 3, pp. 1205-12, (2005) (PubMed).

    Zang, Kalache, Lin, Schroppel, Murphy: "MHC Class II-mediated apoptosis by a nonpolymorphic MHC Class II peptide proceeds by activation of protein kinase C." dans: Journal of the American Society of Nephrology : JASN, Vol. 16, Issue 12, pp. 3661-8, (2005) (PubMed).

    Kleijmeer, Ramm, Schuurhuis, Griffith, Rescigno, Ricciardi-Castagnoli, Rudensky, Ossendorp, Melief, Stoorvogel, Geuze: "Reorganization of multivesicular bodies regulates MHC class II antigen presentation by dendritic cells." dans: The Journal of cell biology, Vol. 155, Issue 1, pp. 53-63, (2001) (PubMed).

    Clausen, Waldburger, Schwenk, Barras, Mach, Rajewsky, Förster, Reith: "Residual MHC class II expression on mature dendritic cells and activated B cells in RFX5-deficient mice." dans: Immunity, Vol. 8, Issue 2, pp. 143-55, (1998) (PubMed).

    De Souza Leao, Lang, Prina, Hellio, Antoine: "Intracellular Leishmania amazonensis amastigotes internalize and degrade MHC class II molecules of their host cells." dans: Journal of cell science, Vol. 108 ( Pt 10), pp. 3219-31, (1995) (PubMed).

    Viville, Neefjes, Lotteau, Dierich, Lemeur, Ploegh, Benoist, Mathis: "Mice lacking the MHC class II-associated invariant chain." dans: Cell, Vol. 72, Issue 4, pp. 635-48, (1993) (PubMed).

    Bhattacharya, Dorf, Springer: "A shared alloantigenic determinant on Ia antigens encoded by the I-A and I-E subregions: evidence for I region gene duplication." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 127, Issue 6, pp. 2488-95, (1982) (PubMed).

  • Antigène
    MHC Class II (MHC2) (Major Histocompatibility Complex Class II Antigen (MHC2))
    Autre désignation
    MHC Class II (MHC2 Produits)
    Synonymes
    anticorps H-2, anticorps MHC-II, anticorps histocompatibility-2, MHC, anticorps H2
    Sujet
    MHC (major histocompatibility complex) class II molecules are transmembrane glycoproteins expressed on the surface of professional antigen-presenting cells, such as macrophages, dendritic cells and B cells. Before their exposition on the cell surface, the MHC class II molecules react with endocytosed exogenous antigens, which are then presented to the T cells. The antigen-binding grove between MHC class II alpha and beta chain is open at both ends and is 15-24 amino acid residues long.
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