ENPP3 anticorps (PE)

N° du produit ABIN457373

Détail du produit anti-ENPP3 anticorps (PE)

Antigène: ENPP3 (Ectonucleotide pyrophosphatase/phosphodiesterase 3 (ENPP3))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp ENPP3 est conjugé à/à la PE

Application: Flow Cytometry (FACS), Immunocytochemistry (ICC)

Specificité: The mouse monoclonal antibody NP4D6 reacts with an extracellular epitope of CD203c, a transmembrane ectoenzyme expressed on basophils and mast cells, and overexpressed upon their activation.

Réactivité croisée (Details): Human

Purification: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogène: HEK-293 cells transfected with human CD203c

Clone: NP4D6

Isotype: IgG1

Information d'application

Indications d'application: Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests. Extracellular and intracellular staining.

Commentaires:

The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

Restrictions: For Research Use only

Stockage

Reconstitution: No reconstitution is necessary.

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze.
Avoid prolonged exposure to light.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Références pour anticorps anti-ENPP3 (PE) (ABIN457373)

Heneberg, Riegerová, Kučera: "Pimecrolimus Is a Potent Inhibitor of Allergic Reactions to Hymenopteran Venom Extracts and Birch Pollen Allergen In Vitro." dans: PLoS ONE, Vol. 10, Issue 11, pp. e0142953, (2015) (PubMed).

Platz, Binder, Marxer, Lischka, Valent, Bühring: "Hymenoptera-venom-induced upregulation of the basophil activation marker ecto-nucleotide pyrophosphatase/phosphodiesterase 3 in sensitized individuals." dans: International archives of allergy and immunology, Vol. 126, Issue 4, pp. 335-42, (2002) (PubMed).

Bühring, Seiffert, Giesert, Marxer, Kanz, Valent, Sano: "The basophil activation marker defined by antibody 97A6 is identical to the ectonucleotide pyrophosphatase/phosphodiesterase 3." dans: Blood, Vol. 97, Issue 10, pp. 3303-5, (2001) (PubMed).

Bühring, Simmons, Pudney, Müller, Jarrossay, van Agthoven, Willheim, Brugger, Valent, Kanz: "The monoclonal antibody 97A6 defines a novel surface antigen expressed on human basophils and their multipotent and unipotent progenitors." dans: Blood, Vol. 94, Issue 7, pp. 2343-56, (1999) (PubMed).

Détails sur ENPP3

Antigène: ENPP3 (Ectonucleotide pyrophosphatase/phosphodiesterase 3 (ENPP3))

Autre désignation: CD203c (ENPP3 Produits)

Synonymes: anticorps B10, anticorps CD203c, anticorps NPP3, anticorps PD-IBETA, anticorps PDNP3, anticorps Npp3, anticorps RB13-6, anticorps AI876438, anticorps ENPP3, anticorps ectonucleotide pyrophosphatase/phosphodiesterase 3, anticorps ectonucleotide pyrophosphatase/phosphodiesterase 3 L homeolog, anticorps ENPP3, anticorps Enpp3, anticorps enpp3, anticorps enpp3.L

Sujet: Ectonucleotide pyrophosphatase/phosphodiesterase 3,CD203c, also known as ENPP-3, is integral membrane ectoenzyme (ectonucleotide pyrophosphatase/phosphodiesterase 3), that hydrolyses nucleotide triphosphates and thus modulates purinergic signaling. CD203c is expressed mainly on activated basophils and mast cells. CD203c is upregulated in response to IgE-receptor cross-linking and is overexpressed on neoplastic mast cells in patients with systemic mastocytosis. Measurement of its induced enhancement on the plasma membrane is useful for diagnostics of allergies.,ENPP3, B10, PDNP3, PD-IBETA

ID gène: 5169

UniProt: O14638

Pathways: Regulation of Muscle Cell Differentiation, Negative Regulation of Transporter Activity