CD86 anticorps

N° du produit ABIN457401

Détail du produit anti-CD86 anticorps

Antigène: CD86

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp CD86 est non-conjugé

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func), Immunocytochemistry (ICC)

Specificité: The rat monoclonal antibody GL-1 reacts with an extracellular epitope of CD86 (B7-2), a 70-80 kDa type I transmembrane glycoprotein of immunoglobulin supergene family, expressed on professional antigen-presenting cells, such as dendritic cells, macrophages or activated B lymphocytes.

Réactivité croisée (Details): Mouse

Purification: Purified by protein-G affinity chromatography.

Pureté: > 95 % (by SDS-PAGE)

niveau d'endotoxine: Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.

Immunogène: LPS-activated CBA/Cs mouse splenic B cells

Clone: GL-1

Isotype: IgG2a kappa

Information d'application

Indications d'application: Functional application: Blocking.
Flow cytometry: Recommended dilution: 2 μg/mL, positive control: murine splenocytes.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4

Agent conservateur: Azide free

Conseil sur la manipulation: Do not freeze.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Do not freeze.

Références pour anticorps anti-CD86 (ABIN457401)

Nolan, Kobayashi, Naveed, Kelly, Hoshino, Hoshino, Karulf, Rom, Weiden, Gold: "Differential role for CD80 and CD86 in the regulation of the innate immune response in murine polymicrobial sepsis." dans: PLoS ONE, Vol. 4, Issue 8, pp. e6600, (2009) (PubMed).

Edgtton, Kausman, Li, OSullivan, Lo, Hutchinson, Yagita, Holdsworth, Kitching: "Intrarenal antigens activate CD4+ cells via co-stimulatory signals from dendritic cells." dans: Journal of the American Society of Nephrology : JASN, Vol. 19, Issue 3, pp. 515-26, (2008) (PubMed).

Nolan, Weiden, Kelly, Hoshino, Hoshino, Mehta, Gold: "CD40 and CD80/86 act synergistically to regulate inflammation and mortality in polymicrobial sepsis." dans: American journal of respiratory and critical care medicine, Vol. 177, Issue 3, pp. 301-8, (2008) (PubMed).

Steptoe, Ritchie, Jones, Harrison: "Autoimmune diabetes is suppressed by transfer of proinsulin-encoding Gr-1+ myeloid progenitor cells that differentiate in vivo into resting dendritic cells." dans: Diabetes, Vol. 54, Issue 2, pp. 434-42, (2005) (PubMed).

Chung, Wells, Adler, Jacob, Turka, Monroe: "Incomplete activation of CD4 T cells by antigen-presenting transitional immature B cells: implications for peripheral B and T cell responsiveness." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 171, Issue 4, pp. 1758-67, (2003) (PubMed).

Brasel, De Smedt, Smith, Maliszewski: "Generation of murine dendritic cells from flt3-ligand-supplemented bone marrow cultures." dans: Blood, Vol. 96, Issue 9, pp. 3029-39, (2000) (PubMed).

Benschop, Melamed, Nemazee, Cambier: "Distinct signal thresholds for the unique antigen receptor-linked gene expression programs in mature and immature B cells." dans: The Journal of experimental medicine, Vol. 190, Issue 6, pp. 749-56, (1999) (PubMed).

Hathcock, Laszlo, Dickler, Bradshaw, Linsley, Hodes: "Identification of an alternative CTLA-4 ligand costimulatory for T cell activation." dans: Science (New York, N.Y.), Vol. 262, Issue 5135, pp. 905-7, (1993) (PubMed).

Détails sur CD86

Antigène: CD86

Autre désignation: CD86 (CD86 Produits)

Synonymes: anticorps B7-2, anticorps B7.2, anticorps B70, anticorps CD28LG2, anticorps LAB72, anticorps B7, anticorps CLS1, anticorps Cd28l2, anticorps ETC-1, anticorps Ly-58, anticorps Ly58, anticorps MB7, anticorps MB7-2, anticorps TS/A-2, anticorps CD86 molecule, anticorps CD86 antigen, anticorps integrin subunit alpha 2b, anticorps CD86, anticorps Cd86, anticorps Itga2b

Sujet: CD86 Molecule,CD80 (B7-1) and CD86 (B7-2) are ligands of T cell critical costimulatory molecule CD28 and of an inhibitory receptor CTLA-4 (CD152). The both B7 Molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, the both molecules can also substitute for each other in this process. The question what are the differences in CD80 and CD86 competency has not been fully elucidated yet, there are still conflicts in results about their respective roles in initiation or sustaining of the T cell immune response.,B7-2, FUN-1, Ly58

ID gène: 12524

UniProt: P42082

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation