ITGAL anticorps

N° du produit ABIN457403

Détail du produit anti-ITGAL anticorps

Antigène: ITGAL (Integrin alpha-L (ITGAL))

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp ITGAL est non-conjugé

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Functional Studies (Func), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificité: The rat monoclonal antibody M17/4 reacts with an extracellular epitope of CD11a (alpha-subunit of murine LFA-1), a 180 kDa type I transmembrane glycoprotein expressed on B and T lymphocytes, monocytes, macrophages, neutrophils, basophils and eosinophils.

Réactivité croisée (Details): Mouse

Purification: Purified by protein-G affinity chromatography.

Pureté: > 95 % (by SDS-PAGE)

niveau d'endotoxine: Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.

Immunogène: C57BL/6 mouse splenic secondary cytotoxic T lymphocytes

Clone: M17-4

Isotype: IgG2a

Information d'application

Indications d'application: Functional application: Blocking.
Flow cytometry: Recommended dilution: 1 μg/mL.
Immunohistochemistry (frozen sections): Positive tissue: murine spleen or thymus, acetone fixation.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4

Agent conservateur: Azide free

Conseil sur la manipulation: Do not freeze.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Do not freeze.

Références pour anticorps anti-ITGAL (ABIN457403)

Smith, Barnum: "Differential expression of beta 2-integrins and cytokine production between gammadelta and alphabeta T cells in experimental autoimmune encephalomyelitis." dans: Journal of leukocyte biology, Vol. 83, Issue 1, pp. 71-9, (2007) (PubMed).

Angelov, Guillaume, Cebecauer, Bosshard, Dojcinovic, Baumgaertner, Luescher: "Soluble MHC-peptide complexes containing long rigid linkers abolish CTL-mediated cytotoxicity." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 176, Issue 6, pp. 3356-65, (2006) (PubMed).

Beyer, Wang, Peters, Doths, Koerner-Rettberg, Openshaw, Schwarze: "The beta2 integrin CD11c distinguishes a subset of cytotoxic pulmonary T cells with potent antiviral effects in vitro and in vivo." dans: Respiratory research, Vol. 6, pp. 70, (2005) (PubMed).

Koseki, Miura, Fujimori, Hokari, Komoto, Hara, Ogino, Nagata, Goto, Hachimura, Kaminogawa, Ishii: "In situ demonstration of intraepithelial lymphocyte adhesion to villus microvessels of the small intestine." dans: International immunology, Vol. 13, Issue 9, pp. 1165-74, (2001) (PubMed).

Papayannopoulou, Priestley, Nakamoto, Zafiropoulos, Scott, Harlan: "Synergistic mobilization of hemopoietic progenitor cells using concurrent beta1 and beta2 integrin blockade or beta2-deficient mice." dans: Blood, Vol. 97, Issue 5, pp. 1282-8, (2001) (PubMed).

Sanchez-Madrid, Davignon, Martz, Springer: "Antigens involved in mouse cytolytic T-lymphocyte (CTL)-mediated killing: functional screening and topographic relationship." dans: Cellular immunology, Vol. 73, Issue 1, pp. 1-11, (1983) (PubMed).

Détails sur ITGAL

Antigène: ITGAL (Integrin alpha-L (ITGAL))

Autre désignation: CD11a (ITGAL Produits)

Synonymes: anticorps CD11A, anticorps LFA-1, anticorps LFA1A, anticorps (p180), anticorps Cd11a, anticorps LFA-1A, anticorps Ly-15, anticorps Ly-21, anticorps CD11a, anticorps ITGAL, anticorps integrin subunit alpha L, anticorps integrin alpha L, anticorps ITGAL, anticorps Itgal

Sujet: Integrin subunit alpha L,CD11a (LFA-1 alpha) together with CD18 constitute leukocyte function-associated antigen 1 (LFA-1), the alphaLbeta2 integrin. CD11a is implicated in activation of LFA-1 complex. LFA-1 is expressed on the plasma membrane of leukocytes in a low-affinity conformation. Cell stimulation by chemokines or other signals leads to induction the high-affinity conformation, which supports tight binding of LFA-1 to its ligands, the intercellular adhesion molecules ICAM-1, -2, -3. LFA-1 is thus involved in interaction of various immune cells and in their tissue-specific settlement, but participates also in control of cell differentiation and proliferation and of T-cell effector functions. Blocking of LFA-1 function by specific antibodies or small molecules has become an important therapeutic approach in treatment of multiple inflammatory diseases. For example, humanized anti-LFA-1 antibody Efalizumab (Raptiva) is being used to interfere with T cell migration to sites of inflammation, binding of cholesterol-lowering drug simvastatin to CD11a allosteric site leads to immunomodulation and increase in lymphocytic cholinergic activity.,LFA-1, LFA1A, ITGAL

ID gène: 16408

UniProt: E9Q5M7

Pathways: Activated T Cell Proliferation, Integrin Complex