Integrin beta 2 anticorps

N° du produit ABIN457404

Détail du produit anti-Integrin beta 2 anticorps

Antigène: Integrin beta 2 (ITGB2)

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp Integrin beta 2 est non-conjugé

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Functional Studies (Func), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificité: The rat monoclonal antibody M18/2 recognizes an extracellular epitope of CD18 antigen (integrin beta2 subunit, beta2 integrin), a 95 kDa type I transmembrane protein expressed on all leukocytes.

Aucune reactivité croisée: Chien

Réactivité croisée (Details): Mouse

Purification: Purified by protein-G affinity chromatography.

Pureté: > 95 % (by SDS-PAGE)

niveau d'endotoxine: Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.

Immunogène: Murine cytotoxic T cell glycoproteins

Clone: M18-2

Isotype: IgG2a

Information d'application

Indications d'application: Functional application: Blocking or stimulation.
Flow cytometry: Recommended dilution: 1 μg/mL.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4

Agent conservateur: Azide free

Conseil sur la manipulation: Do not freeze.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Do not freeze.

Références pour anticorps anti-Integrin beta 2 (ABIN457404)

Cullere, Lauterbach, Tsuboi, Mayadas: "Neutrophil-selective CD18 silencing using RNA interference in vivo." dans: Blood, Vol. 111, Issue 7, pp. 3591-8, (2008) (PubMed).

Varga, Balkow, Wild, Stadtbaeumer, Krummen, Rothoeft, Higuchi, Beissert, Wethmar, Scharffetter-Kochanek, Vestweber, Grabbe: "Active MAC-1 (CD11b/CD18) on DCs inhibits full T-cell activation." dans: Blood, Vol. 109, Issue 2, pp. 661-9, (2007) (PubMed).

Watts, Beurskens, Martin-Padura, Ballantyne, Klickstein, Brenner, Lee: "Manifestations of inflammatory arthritis are critically dependent on LFA-1." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 174, Issue 6, pp. 3668-75, (2005) (PubMed).

Barlow, Langston, Matthews, Chidlow, Kevil: "CD18 deficiency protects against multiple low-dose streptozotocin-induced diabetes." dans: The American journal of pathology, Vol. 165, Issue 6, pp. 1849-52, (2004) (PubMed).

Sakurai, Taguchi, Anand, Ambati, Gragoudas, Miller, Adamis, Ambati: "Targeted disruption of the CD18 or ICAM-1 gene inhibits choroidal neovascularization." dans: Investigative ophthalmology & visual science, Vol. 44, Issue 6, pp. 2743-9, (2003) (PubMed).

Abraham, Miller: "Molecular mechanisms of IL-2 gene regulation following costimulation through LFA-1." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 9, pp. 5193-201, (2001) (PubMed).

Avni, Pur, Yefenof, Baniyash: "Complement receptor 3 of macrophages is associated with galectin-1-like protein." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 12, pp. 6151-8, (1998) (PubMed).

Zahalka, Naor: "Beta 2-integrin dependent aggregate formation between LB T cell lymphoma and spleen cells: assessment of correlation with spleen invasiveness." dans: International immunology, Vol. 6, Issue 6, pp. 917-24, (1994) (PubMed).

Zahalka, Okon, Naor: "Blocking lymphoma invasiveness with a monoclonal antibody directed against the beta-chain of the leukocyte adhesion molecule (CD18)." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 150, Issue 10, pp. 4466-77, (1993) (PubMed).

Sanchez-Madrid, Simon, Thompson, Springer: "Mapping of antigenic and functional epitopes on the alpha- and beta-subunits of two related mouse glycoproteins involved in cell interactions, LFA-1 and Mac-1." dans: The Journal of experimental medicine, Vol. 158, Issue 2, pp. 586-602, (1983) (PubMed).

Détails sur Integrin beta 2

Antigène: Integrin beta 2 (ITGB2)

Autre désignation: CD18 (ITGB2 Produits)

Synonymes: anticorps CD18, anticorps LAD, anticorps LCAMB, anticorps LFA-1, anticorps MAC-1, anticorps MF17, anticorps MFI7, anticorps 2E6, anticorps AI528527, anticorps Cd18, anticorps Lfa1, anticorps ITGB2, anticorps itgb2, anticorps itgb2-a, anticorps itgb2-b, anticorps integrin subunit beta 2, anticorps integrin beta 2, anticorps integrin subunit beta 2 S homeolog, anticorps ITGB2, anticorps Itgb2, anticorps itgb2.S

Sujet: Integrin subunit beta 2,CD18, integrin beta2 subunit, forms heterodimers with four types of CD11 Molecule to constitute leukocyte (beta2) integrins: alphaLbeta2 (CD11a/CD18, LFA-1), alphaMbeta2 (CD11b/CD18, Mac-1, CR3), alphaXbeta2 (CD11c/CD18) and alphaDbeta2 (CD11d/CD18). In most cases, the response mediated by the integrin is a composite of the functions of its individual subunits. These integrins are essential for proper leukocyte migration, mediating intercellular contacts. Absence of CD18 leads to leukocyte adhesion deficiency-1, severe reduction of CD18 expression leads to the development of a psoriasiform skin disease. CD18 is also a target of Mannheimia (Pasteurella) haemolytica leukotoxin and is sufficient to mediate leukotoxin-mediated cytolysis.,Integrin beta2, Complement receptor C3 subunit beta, ITGB2

ID gène: 16414

UniProt: P11835

Pathways: Signalisation NF-kappaB, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation