Ly6g anticorps

N° du produit ABIN457407

Détail du produit anti-Ly6g anticorps

Antigène: Ly6g (Lymphocyte Antigen 6 Complex, Locus G (Ly6g))

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp Ly6g est non-conjugé

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificité: The rat monoclonal antibody RB6-8C5 detects Ly6G component of Gr-1 antigen, a commonly used surface marker of neutrophils.

Réactivité croisée (Details): Mouse

Purification: Purified by protein-A affinity chromatography.

Pureté: > 95 % (by SDS-PAGE)

niveau d'endotoxine: Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.

Immunogène: Murine granulocytes

Clone: RB6-8C5

Isotype: IgG2b

Information d'application

Indications d'application: Functional application: Neutrophil depletion.
Immunoprecipitation: Recommended dilution: 1-2 μg / 100-500 μg of protein.
Western blotting: Recommended dilution: 1 μg/mL.
Flow cytometry: Recommended dilution: 1-4 μg/mL

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4

Agent conservateur: Azide free

Conseil sur la manipulation: Do not freeze.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Do not freeze.

Références pour anticorps anti-Ly6g (ABIN457407)

Jung, Zindl, Lai, Weaver, Chaplin: "MMP induced by Gr-1+ cells are crucial for recruitment of Th cells into the airways." dans: European journal of immunology, Vol. 39, Issue 8, pp. 2281-92, (2009) (PubMed).

dos Santos, Vaz Cardoso, Nascimento, Lino, Dorta, de Oliveira, Ribeiro-Dias: "Leishmania major: recruitment of Gr-1+ cells into draining lymph nodes during infection is important for early IL-12 and IFN gamma production." dans: Experimental parasitology, Vol. 119, Issue 3, pp. 403-10, (2008) (PubMed).

Leendertse, Willems, Giebelen, Roelofs, Bonten, van der Poll: "Neutrophils are essential for rapid clearance of Enterococcus faecium in mice." dans: Infection and immunity, Vol. 77, Issue 1, pp. 485-91, (2008) (PubMed).

Fan, Li, Levy, Fan, Hackam, Vodovotz, Yang, Tracey, Billiar, Wilson: "Hemorrhagic shock induces NAD(P)H oxidase activation in neutrophils: role of HMGB1-TLR4 signaling." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 178, Issue 10, pp. 6573-80, (2007) (PubMed).

Koo, Gan: "The innate interferon gamma response of BALB/c and C57BL/6 mice to in vitro Burkholderia pseudomallei infection." dans: BMC immunology, Vol. 7, pp. 19, (2006) (PubMed).

Nagendra, Schlueter: "Absence of cross-reactivity between murine Ly-6C and Ly-6G." dans: Cytometry. Part A : the journal of the International Society for Analytical Cytology, Vol. 58, Issue 2, pp. 195-200, (2004) (PubMed).

Brown, Blaho, Loiacono: "Susceptibility to experimental Lyme arthritis correlates with KC and monocyte chemoattractant protein-1 production in joints and requires neutrophil recruitment via CXCR2." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 171, Issue 2, pp. 893-901, (2003) (PubMed).

Hestdal, Ruscetti, Ihle, Jacobsen, Dubois, Kopp, Longo, Keller: "Characterization and regulation of RB6-8C5 antigen expression on murine bone marrow cells." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 147, Issue 1, pp. 22-8, (1991) (PubMed).

Détails sur Ly6g

Antigène: Ly6g (Lymphocyte Antigen 6 Complex, Locus G (Ly6g))

Autre désignation: Ly6G (Ly6g Produits)

Synonymes: anticorps Gr-1, anticorps Gr1, anticorps Ly-6G, anticorps lymphocyte antigen 6 complex, locus G, anticorps Ly6g

Sujet: Lymphocyte antigen 6 complex, locus G,Ly6G is a component of the myeloid differentiation antigen Gr-1, together with Ly6C. Ly6G is a good marker for detection of peripheral neutrophils. Expression of Gr-1 in bone marrow correlates with granulocyte differentiation and maturation. Physiological role of Ly6G remains still unclear. Its treatment with antibodies in vivo leads to neutropenia and has inhibitory effect on local immune responses.,Gr1, Gr-1, Ly-6G

ID gène: 546644

UniProt: A0A087WQF5