Actin anticorps

N° du produit ABIN457424

Détail du produit anti-Actin anticorps

Antigène: Actin (ACTA1) (Actin, alpha 1, Skeletal Muscle (ACTA1))

Reactivité: Humain, Souris, Rat, Lapin, Poulet, Chien, Cat, Non-Human Primate

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Actin est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificité: The mouse monoclonal antibody HHF35 recognizes muscle-specific alpha and gamma actin (42 kDa) in various species. This antibody stains skeletal, smooth and myocardial cells as well as myoepithelial cells and pericytes of small vessels. It is a widely used marker of muscle and muscle-derived cells.

Réactivité croisée (Details): Human, Non-Human Primates, Mouse, Rat, Canine (Dog), Feline (Cat), Rabbit, Chicken

Purification: Purified by protein-A affinity chromatography.

Pureté: > 95 % (by SDS-PAGE)

Immunogène: SDS extracted protein fraction of human myocardium

Clone: HHF35

Isotype: IgG1

Information d'application

Indications d'application: Western blotting: Recommended dilution: 1 μg/mL, positive control: murine femoral muscle, murine heart, negative control: HUVEC line, reducing conditions.
Immunohistochemistry (paraffin sections): Antigen retrieval steps generally not required, but e.g. in case of arterial smooth muscle cells or myoepithelial cells, pepsin or trypsin pretreatment is recommended.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Do not freeze.

Références pour anticorps anti-Actin (ABIN457424)

Matsuo, Susumu, Tsutsumi, Azuma, Obata, Hayashi: "Glomus tumor of the omentum: a case report." dans: Journal of surgical oncology, Vol. 96, Issue 7, pp. 633-6, (2007) (PubMed).

Matsuyama, Hisaoka, Hashimoto: "Angioleiomyoma: a clinicopathologic and immunohistochemical reappraisal with special reference to the correlation with myopericytoma." dans: Human pathology, Vol. 38, Issue 4, pp. 645-51, (2007) (PubMed).

Ijzer, Roskams, Molenbeek, Ultee, Penning, Rothuizen, van den Ingh: "Morphological characterisation of portal myofibroblasts and hepatic stellate cells in the normal dog liver." dans: Comparative hepatology, Vol. 5, pp. 7, (2006) (PubMed).

Handharyani, Ochiai, Iwata, Umemura: "Immunohistochemical and ultrastructural study of ito cells (fat-storing cells) in response to extrahepatic bile duct ligation in broiler chickens." dans: The Journal of veterinary medical science / the Japanese Society of Veterinary Science, Vol. 63, Issue 5, pp. 547-52, (2001) (PubMed).

de Sousa, Schwarzschild, de Araújo, de Araújo: "Basal cell adenocarcinoma of the palate with squamous metaplasia." dans: Journal of clinical pathology, Vol. 53, Issue 2, pp. 153-6, (2000) (PubMed).

Miettinen: "Antibody specific to muscle actins in the diagnosis and classification of soft tissue tumors." dans: The American journal of pathology, Vol. 130, Issue 1, pp. 205-15, (1988) (PubMed).

Schmidt, Cone, Haas, Gown: "Diagnosis of rhabdomyosarcomas with HHF35, a monoclonal antibody directed against muscle actins." dans: The American journal of pathology, Vol. 131, Issue 1, pp. 19-28, (1988) (PubMed).

Tsukada, McNutt, Ross, Gown: "HHF35, a muscle actin-specific monoclonal antibody. II. Reactivity in normal, reactive, and neoplastic human tissues." dans: The American journal of pathology, Vol. 127, Issue 2, pp. 389-402, (1987) (PubMed).

Tsukada, Tippens, Gordon, Ross, Gown: "HHF35, a muscle-actin-specific monoclonal antibody. I. Immunocytochemical and biochemical characterization." dans: The American journal of pathology, Vol. 126, Issue 1, pp. 51-60, (1987) (PubMed).

Détails sur Actin

Antigène: Actin (ACTA1) (Actin, alpha 1, Skeletal Muscle (ACTA1))

Autre désignation: Actin (muscle) (ACTA1 Produits)

Synonymes: anticorps ACTA, anticorps ASMA, anticorps CFTD, anticorps CFTD1, anticorps CFTDM, anticorps MPFD, anticorps NEM1, anticorps NEM2, anticorps NEM3, anticorps acta, anticorps asma, anticorps cftd, anticorps cftd1, anticorps cftdm, anticorps mpfd, anticorps nem1, anticorps AA959943, anticorps Acta-2, anticorps Acts, anticorps Actsk-1, anticorps actc, anticorps actc1, anticorps cfk, anticorps hm:zeh0631, anticorps zeh0631, anticorps acta1, anticorps hm:zeh0293, anticorps zgc:112098, anticorps actin, alpha 1, skeletal muscle, anticorps actin, alpha 1, skeletal muscle L homeolog, anticorps actin, alpha 1b, skeletal muscle, anticorps actin, muscle, anticorps actin, alpha skeletal muscle, anticorps actin, alpha 1a, skeletal muscle, anticorps ACTA1, anticorps acta1.L, anticorps acta1, anticorps Acta1, anticorps acta1b, anticorps LOC100533357, anticorps LOC109479779, anticorps LOC109108280, anticorps acta1a

Sujet: Actin is a highly conserved ubiquitous globular protein (G-actin) that polymerizes to form fibrous F-actin microfilaments. In higher eucaryotes several actin isoforms have been identified, that fall into three classes. Alpha actin is a structural component of the contractile apparatus of muscle cells or muscle-derived cells. Beta actin and gamma actin play roles in regulation of cell motility in other cell types. Specific subcellular structures such as stress fibers, focal adhesions, filopodia etc., are formed by involvement of actin cytoskeleton.,Muscle specific actin

Pathways: Caspase Cascade in Apoptosis, Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development