Ovalbumin anticorps (FITC)

N° du produit ABIN457639

Détail du produit anti-Ovalbumin anticorps (FITC)

Antigène: Ovalbumin (OVA)

Reactivité: Poulet

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Ovalbumin est conjugé à/à la FITC

Application: Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Specificité: Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins, since homologous proteins of different species frequently share antigenic determinants. of this antiserum has not been tested in detail.

Attributs du produit: Fluorescein isothiocyanate-conjugated IgG fraction of polyclonal rabbit antiserum to chicken ovalbumin

Purification: The IgG (7S) fraction is isolated and purified from hyperimmune antisera with strong precipitating activity and contains the bulk of the antibody specificity. It is free of other serum proteins as tested by immuno-electrophoresis and double radial immunodiffusion.

Immunogène: Highly purified egg albumin (MW 45,000) isolated from chicken egg whites. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Information d'application

Indications d'application: In immunocytochemical and immunohistochemical staining of ovalbumin at the cellular and subcellular level of appropriately treated cell and tissue substrates. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:20 and 1:80, depending on the method used.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Concentration: IgG protein concentration 10 mg/ml. Fluorochrome/IgG protein molar ratio (F/P) is approximately 1.7. No foreign proteins added.

Buffer: FITC-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2).

Agent conservateur: Without preservative

Stock: 4 °C/-20 °C

Stockage commentaire: The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconstituted by ad ding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.

Détails sur Ovalbumin

Antigène: Ovalbumin (OVA)

Autre désignation: Ovalbumin (OVA Produits)

Synonymes: anticorps 1600019A21Rik, anticorps R86, anticorps SPI-CI, anticorps Spi10, anticorps ovalbumin, anticorps NK13, anticorps Spi12, anticorps BC052216, anticorps NK21, anticorps NK21L1, anticorps Spi13, anticorps NK26, anticorps Spi14, anticorps AW540195, anticorps AT2, anticorps Spi9, anticorps EID, anticorps SPI3B, anticorps Gm11396, anticorps OTTMUSG00000000720, anticorps SERPINB14, anticorps C76171, anticorps C76174, anticorps SPI3C, anticorps SPIC, anticorps serine (or cysteine) peptidase inhibitor, clade B, member 9b, anticorps serine (or cysteine) peptidase inhibitor, clade B, member 6b, anticorps serine (or cysteine) peptidase inhibitor, clade B, member 9f, anticorps serine (or cysteine) peptidase inhibitor, clade B, member 9e, anticorps serine (or cysteine) peptidase inhibitor, clade B, member 9d, anticorps serine (or cysteine) peptidase inhibitor, clade B, member 1, pseudogene, anticorps serine (or cysteine) peptidase inhibitor, clade B, member 6e, anticorps ovalbumin (SERPINB14), anticorps serine (or cysteine) peptidase inhibitor, clade B, member 6c, anticorps Serpinb9b, anticorps Serpinb6b, anticorps Serpinb9f, anticorps Serpinb9e, anticorps Serpinb9d, anticorps Serpinb1-ps1, anticorps Serpinb6e, anticorps OVAL, anticorps Serpinb6c

Sujet: A single precipitin line is obtained in immunoelectrophoresis against 1% chicken egg albumin in PBS. In double radial immunodiffusion of the antiserum against twofold dilutions of 1% chicken egg albumin in PBS and in the reverse arrangement of the chicken egg albumin solution against twofold dilutions of the antiserum, a singe precipitin line is obtained. The antiserum does not react with chicken serum