PF4 anticorps
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- Antigène Voir toutes PF4 Anticorps
- PF4 (Platelet Factor 4 (PF4))
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Reactivité
- Humain
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp PF4 est non-conjugé
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Application
- ELISA, Immunoelectrophoresis (IEP), Immunodiffusion (ID), Radial Immunodiffusion (RID), Nephelometry (Neph)
- Specificité
- Inter-species cross-reactivity is a normal feature of antibodies to serum proteins, since homologous proteins of different species frequently share antigenic determinants. of this antiserum has not been tested in detail. In double radial immunodiffusion a reaction has been observed with bovine, cat, dog and monkey.
- Attributs du produit
- Precipitating polyclonal rabbit antiserum to human platelet factor 4
- Purification
- Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.
- Immunogène
- Platelet factor 4 is a low molecular weight (MW 7,800) heat stable protein, secreted by alpha granules of the platelets. Purified platelet factor 4 isolated from pooled human serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
- Isotype
- IgG
- Top Product
- Discover our top product PF4 Anticorps primaire
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- Indications d'application
- In precipitating techniques as electroimmunodiffusion (Laurell), immunoelectrophoresis and single and double radial immunodiffusion to identify the presence platelet factor 4. This antiserum has not been tested in more sensitive assays as nephelometry, ELISA or use in immunohistology, but this does not exclude such use if proper controls are included. Measurement of platelet factor 4 is of significance for diagnosis of platelet disorders involving platelet alpha granules and to demonstrate increased platelet secretion in vivo. Reduced levels are measured in gray platelet syndrome, whereas elevated levels have been reported in cases as coronary artery disease, myocardial infarction, disseminated intravascular coagulation, cerebrovascular disease and cancer.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Concentration
- Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal rabbit serum. No foreign proteins added. Antibody titre: The amount of platelet factor 4 precipitated by 1 ml antiserum is approximately 0.1 mg.
- Buffer
- Delipidated, heat inactivated, lyophilized, stable whole antiserum
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- 4 °C/-20 °C
- Stockage commentaire
- The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Reconstitute the lyophilized antiserum by add ing 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not ref rozen, and preferably used the same day.
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- Antigène
- PF4 (Platelet Factor 4 (PF4))
- Autre désignation
- Platelet Factor 4 (PF4 Produits)
- Synonymes
- anticorps CXCL4, anticorps PF-4, anticorps SCYB4, anticorps Cxcl4, anticorps Scyb4, anticorps Pf4a, anticorps RATPF4A, anticorps PF4A, anticorps CXCL4L1, anticorps CXCL4V1, anticorps PF4-ALT, anticorps SCYB4V1, anticorps PF4, anticorps platelet factor 4, anticorps platelet factor 4 variant 1, anticorps PF4, anticorps Pf4, anticorps LOC703451, anticorps PF4V1, anticorps LOC100524373, anticorps LOC102181854, anticorps LOC100630489
- Sujet
- The reactivity of the antiserum is restricted to platelet factor 4 as tested in immunoelectro-phoresis and radial immunodiffusion. A characteristic single precipitin line is obtained with normal serum which shows a reaction of identity with the precipitin line with purified immunogen
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