Secretory Component anticorps

N° du produit ABIN458282

Détail du produit anti-Secretory Component anticorps

Antigène: Secretory Component

Reactivité: Singe

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp Secretory Component est non-conjugé

Application: Immunoelectrophoresis (IEP)

Specificité: Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Precipitation reactions have been observed with free and bound secretory component in serum of other old-world monkeys, including Cercopithecus, Cynomolgus and Baboon. The antiserum may also react with other species as has been observed for chimpanzee.

Attributs du produit: Precipitating polyclonal goat antiserum to monkey secretory component

Purification: Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required to eliminate antibody activity to any other serum protein. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.

Immunogène: Secretory component is present in monkey secretions bound to secretory IgA (sIgA) and in free form. Secretory IgA (sIgA) functions as a dimer or polymer and accounts for almost all specific mucosal antibody activity. A molecule of sIgA is made up of two molecules of IgA, one J chain and one SC (MW 65,000). The dimer IgA is transported into secretions by its binding to the SC on the epithelial cells. SC also has an affinity for polymeric IgM. Purified free secretory component isolated from pooled rhesus monkey milk is used for immunization. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Information d'application

Indications d'application: In precipitating techniques as immunoelectrophoresis and single and double radial immuno-diffusion to identify the presence secretory component in monkey serum or other body fluids and to determine its concentration.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Concentration: Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal goat serum. No foreign proteins added. Antibody titre: Precipitin titre 1:64 when tested against pooled normal monkey milk in agar-block immuno-diffusion titrat

Buffer: Delipidated, heat inactivated, lyophilized, stable whole antiserum

Agent conservateur: Without preservative

Stock: 4 °C/-20 °C

Stockage commentaire: The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Reconstitute the lyophilized antiserum by add ing 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not ref rozen, and preferably used the same day.

Références pour anticorps anti-Secretory Component (ABIN458282)

Thomas, Tuero, Demberg, Vargas-Inchaustegui, Musich, Xiao, Venzon, LaBranche, Montefiori, DiPasquale, Reed, DeVico, Fouts, Lewis, Gallo, Robert-Guroff: "HIV-1 CD4-induced (CD4i) gp120 epitope vaccines promote B and T-cell responses that contribute to reduced viral loads in rhesus macaques." dans: Virology, Vol. 471-473, pp. 81-92, (2015) (PubMed).

Détails sur Secretory Component

Antigène: Secretory Component

Abstract: Secretory Component Produits

Synonymes: anticorps RNPIGR2, anticorps pIgA-R, anticorps polymeric immunoglobulin receptor, anticorps PIGR, anticorps Pigr

Sujet: Tested in immunoelectrophoresis, double radial immunodiffusion and ELISA against a panel of appropriate secretions and purified Ig isotypes. The antiserum reacts with both bound secretory component (secretory IgA) and with the free SC present in monkey secretions. In immuno-electrophoresis against monkey milk, using a high electroendosmosis agar plate, free SC is precipitated in the alph-2 region. The antiserum does not react with other molecular forms of IgA, or with any other secretory or plasma protein.