Fibrinogen anticorps

N° du produit ABIN458504

Détail du produit anti-Fibrinogen anticorps

Antigène: Fibrinogen

Reactivité: Souris

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp Fibrinogen est non-conjugé

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificité: The antiserum does not cross-react with any other component of mouse plasma. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. of this antiserum has not been tested in detail.

Attributs du produit: Purified IgG fraction of polyclonal goat antiserum to mouse fibrinogen

Purification: Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies cross-reacting with other with other plasma proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum. The IgG (7S) fraction is isolated and purified from the antiserum and contains the bulk of the defined antibody specificity. It is free of other serum proteins as tested by immunoelectrophoresis and double radial immunodiffusion.

Immunogène: Fibrinogen (clotting factor I) is a heat labile beta glycoprotein present in plasma. It is the precursor of fibrin, which is the key protein constituting the network of the blood clot. Thrombin converts fibrinogen to fibrin by limited proteolysis. Fibrin monomers polymerize to fibrin which is stabilized by cross-linking. Fibrinogen is isolated from fresh plasma after removing prothrombin. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Information d'application

Indications d'application: As unlabelled primary or secondary antibody reagent for the indirect detection of fibrinogen in mouse cells, tissues and body fluids in immunofluorescence and immunoenzyme methods, for the production of immunoconjugates with a selected marker, to prepare insoluble immunoaffinity adsorbents by coupling to an artificial carrier, as catching or detection reagent in non-isotopic methodology and solid phase immunochemistry (e.g. ELISA, Western blotting). When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration. Typical working dilutions in histochemistry are usually between 1:50 and 1:250, in ELISA and comparable non-precipitating antibody-binding assays between 1:500 and 1:5,000.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Concentration: Physicochemical characteristic IgG protein concentration 10 mg/ml. No foreign proteins added.

Buffer: Purified hyperimmune IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2)

Agent conservateur: Without preservative

Stock: 4 °C

Références pour anticorps anti-Fibrinogen (ABIN458504)

Zang, Gustafsson, Jamalpour, Hong, Genové, Welsh: "Vascular dysfunction and increased metastasis of B16F10 melanomas in Shb deficient mice as compared with their wild type counterparts." dans: BMC cancer, Vol. 15, pp. 234, (2016) (PubMed).

Tugues, Roche, Noguer, Orlova, Bhoi, Padhan, Akerud, Honjo, Selvaraju, Mazzone, Tolmachev, Claesson-Welsh: "Histidine-rich glycoprotein uptake and turnover is mediated by mononuclear phagocytes." dans: PLoS ONE, Vol. 9, Issue 9, pp. e107483, (2014) (PubMed).

Détails sur Fibrinogen

Antigène: Fibrinogen

Abstract: Fibrinogen Produits

Synonymes: anticorps fib2, anticorps MGC53620, anticorps fibrinogen, anticorps sb:cb892, anticorps wu:fa55c12, anticorps wu:fb20e08, anticorps wu:fb59b03, anticorps wu:fi39d04, anticorps zgc:92117, anticorps fibrinogen alpha chain, anticorps fibrinogen gamma chain, anticorps fga, anticorps FGA, anticorps LOC698244, anticorps CpipJ_CPIJ010090, anticorps FGG

Sujet: The reactivity of the antiserum is restricted to fibrinogen. In immunoelectrophoresis and radial immunodiffusion (Ouchterlony), using various antiserum concentrations against normal mouse plasma a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified fibrinogen. No reaction is obtained with any other plasma protein component or serum. However, the antiserum may also react with fibrin monomers, circulating fibrinopeptides and fibrin degradation products