PNP anticorps (Biotin)
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- Antigène Voir toutes PNP (NP) Anticorps
- PNP (NP) (Nucleoside Phosphorylase (NP))
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Reactivité
- Boeuf (Vache)
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp PNP est conjugé à/à la Biotin
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Application
- Western Blotting (WB), ELISA, Immunofluorescence (IF)
- Specificité
- Cross-reactivities against enzymes of other sources may occur but have not been determined.
- Attributs du produit
- Biotin-conjugated IgG fraction of polyclonal rabbit antiserum to nucleoside phosphorylase from calf spleen
- Purification
- The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.
- Immunogène
- Nucleoside phosphorylase isolated and purified from calf spleen. Freund’s complete adjuvant is used in the first step of the immunization procedure.
- Isotype
- IgG
- Top Product
- Discover our top product NP Anticorps primaire
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- Indications d'application
- This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques).
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Concentration
- IgG protein concentration 10 mg/ml. Biotin/IgG protein molar ratio (B/P) approximately 5.0. No foreign proteins added.
- Buffer
- Biotin-coupled hyperimmune rabbit IgG lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2).
- Agent conservateur
- Without preservative
- Stock
- 4 °C/-20 °C
- Stockage commentaire
- The lyophilised conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconsti tuted by adding 1.0 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at a mbient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used t he same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
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- Antigène
- PNP (NP) (Nucleoside Phosphorylase (NP))
- Autre désignation
- Nucleoside Phosphorylase (NP Produits)
- Synonymes
- anticorps NP, anticorps PRO1837, anticorps PUNP, anticorps Np, anticorps AL024301, anticorps AU015798, anticorps Np-1, anticorps Np-2, anticorps Pnp1, anticorps purine nucleoside phosphorylase, anticorps purine-nucleoside phosphorylase, anticorps PNP, anticorps Pnp
- Classe de substances
- Viral Protein
- Sujet
- The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.
- Pathways
- Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Ribonucleoside Biosynthetic Process, Positive Regulation of Response to DNA Damage Stimulus
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