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Enolase anticorps (Biotin)

Reactivité: Lapin ELISA, WB, IF Hôte: Mouton Polyclonal Biotin
N° du produit ABIN459122
  • Antigène Voir toutes Enolase Anticorps
    Enolase
    Reactivité
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Lapin
    Hôte
    • 3
    • 2
    • 1
    Mouton
    Clonalité
    • 3
    • 3
    Polyclonal
    Conjugué
    • 6
    Cet anticorp Enolase est conjugé à/à la Biotin
    Application
    • 5
    • 4
    • 2
    • 2
    ELISA, Western Blotting (WB), Immunofluorescence (IF)
    Specificité
    Cross-reactivities against enzymes of other sources may occur but have not been determined.
    Attributs du produit
    Biotin-conjugated IgG fraction of polyclonal sheep antiserum to enolase from rabbit muscle
    Purification
    The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.
    Immunogène
    Enolase isolated and purified from rabbit muscle. Freund’s complete adjuvant is used in the first step of the immunization procedure.
    Isotype
    IgG
    Top Product
    Discover our top product Enolase Anticorps primaire
  • Indications d'application
    This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immuno-fluorescence or histochemical techniques).
    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Concentration
    IgG protein concentration 10 mg/ml. Biotin/ IgG protein molar ratio (B/P) approximately 6.7. No foreign proteins added.
    Buffer
    Biotin-coupled hyperimmune sheep IgG lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2).
    Agent conservateur
    Without preservative
    Stock
    4 °C/-20 °C
    Stockage commentaire
    The lyophilised conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconstituted by adding 1.0 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at a mbient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used t he same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
  • Antigène
    Enolase
    Abstract
    Enolase Produits
    Synonymes
    anticorps BEST:LD15491, anticorps CG17654, anticorps CT32526, anticorps Dmel\\CG17654, anticorps ENOA, anticorps Eno2, anticorps T18, anticorps anon-WO0153538.76, anticorps eno, anticorps ECK2773, anticorps JW2750, anticorps T22I11.6, anticorps T22I11_6, anticorps Enolase, anticorps enolase, anticorps phosphopyruvate hydratase, anticorps O-methyltransferase family protein, anticorps Eno, anticorps eno, anticorps Dvir\Eno, anticorps eno-4, anticorps MMAH_RS09975, anticorps IGMT3
    Sujet
    The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.
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