FH anticorps
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- Antigène Voir toutes FH Anticorps
- FH (Fumarate Hydratase (FH))
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Reactivité
- Porc
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp FH est non-conjugé
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Application
- Western Blotting (WB), ELISA, Immunofluorescence (IF)
- Specificité
- Cross-reactivities against enzymes of other sources may occur but have not been determined.
- Attributs du produit
- IgG fraction of polyclonal rabbit antiserum to fumarase from porcine heart
- Purification
- The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.
- Immunogène
- Fumarase is isolated and purified from porcine heart. Freund’s complete adjuvant is used in the first step of the immunization procedure.
- Isotype
- IgG
- Top Product
- Discover our top product FH Anticorps primaire
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- Indications d'application
- This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques), to prepare an insoluble immuno-affinity adsorbent, for labelling with a marker of the customer’s own choice.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Concentration
- IgG protein concentration 10 mg/ml. No foreign proteins added.
- Buffer
- Purified hyperimmune rabbit IgG lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2).
- Agent conservateur
- Without preservative
- Stock
- 4 °C/-20 °C
- Stockage commentaire
- The lyophilised IgG fraction is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconstituted by adding 1.0 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at a mbient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used t he same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
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- Antigène
- FH (Fumarate Hydratase (FH))
- Autre désignation
- Fumarase (FH Produits)
- Synonymes
- anticorps FH, anticorps DDBDRAFT_0205237, anticorps DDBDRAFT_0231397, anticorps DDBDRAFT_0231400, anticorps DDB_0205237, anticorps DDB_0231397, anticorps DDB_0231400, anticorps HLRCC, anticorps LRCC, anticorps MCL, anticorps MCUL1, anticorps Fh1, anticorps im:7152785, anticorps ns:zf-e152, anticorps zf-e152, anticorps zgc:66253, anticorps zgc:77498, anticorps Fh, anticorps Fh-1, anticorps fumarate hydratase, anticorps fumarate hydratase 1, anticorps FH, anticorps fumH, anticorps Fh, anticorps fh, anticorps Fh1
- Sujet
- The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.
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