Pyrophosphatase (Inorganic) 1 (PPA1) anticorps

N° du produit ABIN459215

Détail du produit

Antigène: Pyrophosphatase (Inorganic) 1 (PPA1)

Reactivité: Saccharomyces cerevisiae

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Inconjugué

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Specificité: Cross-reactivities against enzymes of other sources may occur but have not been determined.

Attributs du produit: IgG fraction of polyclonal rabbit antiserum to inorganic pyrophosphatase from yeast

Purification: The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.

Immunogène: Inorganic pyrophosphatase is isolated and purified from yeast. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Information d'application

Indications d'application: This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques), to prepare an insoluble immuno-affinity adsorbent, for labelling with a marker of the customer’s own choice.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Concentration: IgG protein concentration 10 mg/ml. No foreign proteins added.

Buffer: Purified hyperimmune rabbit IgG lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2).

Agent conservateur: Without preservative

Stock: 4 °C/-20 °C

Stockage commentaire: The lyophilised IgG fraction is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconsti tuted by adding 1.0 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at a mbient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used t he same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Référence

Patir-Nebioglu, Andrés, Krebs, Fink, Drzewicka, Stankovic-Valentin, Segami, Schuck, Büttner, Hell, Maeshima, Melchior, Schumacher: "Pyrophosphate modulates plant stress responses via SUMOylation." dans: eLife, Vol. 8, (2019) (PubMed).

Détail du antigène

Antigène: Pyrophosphatase (Inorganic) 1 (PPA1)

Autre désignation: Inorganic Pyrophosphatase (PPA1 Produits)

Synonymes: anticorps ppa, anticorps SCE9.16, anticorps PSPTO0722, anticorps AO090005001437, anticorps IOPPP, anticorps PP, anticorps PP1, anticorps SID6-8061, anticorps 2010317E03Rik, anticorps Pyp, anticorps Pp, anticorps inorganic pyrophosphatase, anticorps Inorganic pyrophosphatase, anticorps pyrophosphatase (inorganic) 1 L homeolog, anticorps pyrophosphatase (inorganic) 1, anticorps ppa, anticorps SCO3409, anticorps ppa-1, anticorps APE_1692.1, anticorps AOR_1_2492174, anticorps Celal_2154, anticorps Celly_1958, anticorps Dester_1338, anticorps Marky_0054, anticorps Halhy_5834, anticorps ppa1.L, anticorps PPA1, anticorps Ppa1

Classe de substances: Viral Protein

Sujet: The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.